Study On Carbendazim-resistant Colletotrichum Gloeosporioides (Penz.) Sacc. In Liaoning

Grape anthracnose is one of the important diseases on the grape production in Liaoning Province,have been resistant to carbendazim.This article based on extensive investigation and collection of pathogens,application of the traditional morphology and molecular biology methods for pathogen identification;established baseline- sensitivity of grape anthracnose to carbendazim and determined sensitivity;assessed the risk of carbendazim on the drug-resistant;conducted indoor pharmaceutical screening,so as to provide a theoretical basis to improve the pharmacodynamic of agricultural production,reduce losses and disease prevention.The results obtained are as follows:â… .As the 24 anthrax strains of grapes of the main grape producing areas in Liaoning Province determination of pathogenic,identification of pathogen morphology and further use of rRNA-ITS sequence analysis section for molecular identification,the results show that:Arising from different regions of Liaoning Province of grape anthracnose pathogen was identified as semi-known in the anthrax cotina genus Colletotrichum gloeosporioides (ColIetotrichum gloeosporioides(Penz.) Sacc.).And detected with the ITS sequences in the GenBank nucleic acid data showed that bacteria,than has been published with the Colletotrichum gloeosporioides high homology similarity,identification and biology of the results were consistent.â…¡.Established grape anthrax baseline-sensitivity to carbendazim,the growth rate method for the determination of 24 kinds of grapes in this strain of anthrax carbendazim sensitivity,and results showed that:the sensitivity of bacteria on the determination of carbendazim EC₅₀ value of scope 0.2678μg/mL～2.7312μg/mL,average 1.14492μg/mL. Most of the drug-resistant strains resistant to the level of performance(EC₅₀ for 0.4598μg/mL～2.299μg/mL),high resistance is only one strain(EC₅₀ for 2.7312μg/mL), and different frequency distribution of the sensitivity of the normal distribution was irregular,there is the risk of decreased sensitivity.â…¢.Grape anthrax had indoor risk assessment to carbendazim.The experimental results show that:the use of UV-induced acclimation and pharmaceutical methods of three drug-resistant mutants,resistance level is 6431.96 times the 46.76 percent and 11.72 percent.The two UV-induced mutant strains with natural resistance after subculture through the stability of higher pharmaceutical domesticated strains after subculture by the stability of the larger drop.Comparisons in the pathogenicity,the strains did not pro-induced and natural resistance of the strain of highly pathogenic strains.Determination in terms of fit of the strains in the mycelial growth rate,sporulation and spore germination rate,SJX-U2,SJX-Y1,FML strain than the sensitive strains SJX have a good fit,and SJX-U1 weaker fitness.Pro-competitiveness of this strain is superior to the interior of drug-resistant mutant in the mixed conditions,a certain degree of competitiveness.SJX strain on the Pharmacy and FML no cross-resistance,and the strain induced by SJX-U2 and SJX-Y1 special bacteria to retire there is no cross-resistance,while thiram, chlorothalonil,thiophanate-methyl Pharmacy there are cross-resistant sexual.â…£.On SJX,FML,SJX-Y1 of the three strains to carry out efficacy testing,results showed that:70%thiophanate-methyl WP,50%retired special bacteria WP,50% carbendazim WP,50%amine Prochloraz WP efficacy of four fungicides relatively good inhibitory rate.Taking into account the price,toxins,effects,etc.,for different concentrations of pharmaceutical screening,the results of the sensitive strain with 1,000 times the rate of 50%Carbendazim Bacteriostasis better,with 4000-fold resistant strains 50%better Prochloraz Bacteriostasis amine.