Analysis On Gliadin And HMW-GS Of Some Wheat Cultivars (Lines) In Henan Province

In order to clarify content of gliadin,HMW-GS and genetic diversity of some wheat cultivars (lines) in Henan Province, experiments were carried out in Henan University and Henan Vocational College of Agriculture from 2006 to 2008. A-PAGE and SDS-PAGE were used to analyse genetic diversity of gliadin and composition of HMW-GS in 35 wheat cultivars (lines) in Henan Province. The main results were as follows:1. Some wheat cultivars (lines) in Henan Province have genetic diversity of gliadin detected gliadin gene locus specificity of the grain of some wheat cultivars (lines), analyzed the differences of gliadin-based genetic between the different cultivars (lines). The results showed 53 gliadin bands were separated from 35 materials. 11 materials with a marker of 1BL/1RS translocation with allele Gli-Bll, accounted the test materials for 31.4%. The genetic distance (GD) based on gliadin patterns ranged from 0.0267 to 0.926, with a mean value of 0.5523. Cluster analysis based on genetic distance showed that the studied materials can be classified into 3 clusters at the distance of GD 0.58. It was concluded that some wheat cultivar(slines)in Henan province had a rich genetic diversity based on these results.2. 5+12,2﹡allele of some wheat cultivars (lines) in Henan Province were lower Analyzed composition of HMW-GS 15 HMW-GS of some wheat cultivars (lines), allele's variation were identified on Glu-1 site. Subunits 1,2﹡and Null at Glu-A1 were found with frequencies of 75.0%,8.33% and 16.67%, respectively; subunits 7,7+8,7+9,13+16,13+19,14+15 and 17+18 at Glu-B1 with frequencies of 5.6%, 36.12%, 33.3%, 5.6%, 2.8%, 13.9% and 2.8%, respectively; and subunits 2+12, 2+11, 3+12, 5+10, 5+12 at Glu-D1 with frequencies of 36.1%, 2.78%, 8.3%, 50.0% and 2.78%, respectively. There were 18 types of good subunits among them, which promoted the quality of wheat-breeding, such as 5+12, 5+10, 1, 2﹡, 7+8, 14+15, 17+18, 13+16. These results showed 5+12, 2﹡were low in some wheat cultivars (lines) in Henan Province. Therefore more excellent parents for wheat-breeding should be introduced and exploited in the future.3. HMW-GS can be used as one of important indicators of the quality in classification Comparative Analyzed different types of HMW-GS and strong gluten, Medium Gluten, weak gluten quality 3 types of quality characteristics, and the results are similar to the effects of HMW-GS on quality traits.4. Different types of quality represent of different cultivars (lines) Through the study of the grain gliadin and HMW-GS composition of some wheat cultivars (lines) in Henan Province, Zhengmai 9023, Jimai20, Zhengmai 366 were Strong Gluten Wheat; Yanzhang 4110, Xunong 5, Zhengnong17 were Medium Gluten Wheat; Zhengfeng 5, Jimai 2 were Weak Gluten Wheat.