Avian Influenza Virus Antigen Purification Methods Set Up And Purification Of Antigen To Study The Effect Of The Chicken Immune

In order to adapt to the requirements of large-scale production, the test in order to set up a cloned strain of antigen, antigen pre-processing, low-speed centrifugation impurity, high-speed centrifugal impurity, concentrated, purified antigen-based purification method of the avian flu, to reduce the antigen impurity content in the protein content and miscellaneous. Preparation of vaccine antigen with purified antigen and purification of conventional antigen test in accordance with established methods of emulsion preparation of vaccine, purified vaccine compared with the conventional vaccine is basically the same as the physical appearance of characters, but through the particle size particle size detection of purified vaccine more small bodies absorb more chicken . Antibodies have higher, and maintain longer, about 18 weeks of conventional vaccine is no longer produced antibodies, vaccines made of purified antigen at 20 weeks was maintained at a high level, testing the effectiveness of the vaccine tests show: vaccine tests show the effectiveness of testing: three groups at the first post-vaccination days, each group 10,14,21,40 geometric mean titer levels of antibodies followed by conventional vaccine group: 9.01Log2; purified vaccine antigen b dose group: 8.92 Log2; antigen c Purification of vaccine dose group: 10.42 Log2. Three groups of SPF chickens chest vaccine 1ml by intramuscular injection six days after vaccination, three groups of chickens vaccinated vaccination site are visible to varying degrees vaccine situation has not been completely absorbed, partial purification of the vaccine group 1,2 has swelling along the injection direction scattered bleeding points. The first 25 days autopsy, purified vaccine vaccine 1,2 group basic absorption, better restore muscle tissue, the conventional vaccine group of vaccines have not been completely absorbed in and around the injection site localized slightly yellow viscous liquid, there is a small amount of bleeding points. 9th day of the control group autopsy, the injection site has a slight hyperemia and hemorrhage, but was not obvious, antigen completely absorbed, no abnormal muscle.