Studies On The Technical Of Embryo Culture And Rapid Propagation Of Osmanthus Fragrans 'Zaohuazijinggui' In Vitro

This paper mainly dealt with the study on Osmanthus fragrans 'Zaohuazijinggui' as explants for regenerated plantlets of embryo. By means of two elements design, orthogonal experimental design and statistical analysis method, the affected factors, existing problems of propagation and the relevant solutions were discussed; the technique of tissue culture was established with best culture medium and procedure. The main results were as follows:1. Mid March was the best season for explants taking on Osmanthus fragrans 'Zaohuazijinggui'. The initiation rate could be up to 88.90%.2. Embryo initial cultureThe optimizing medium for embryo initial culture was MS medium with 6-BA 1.50 mg/L, the initiation rate was 90%, and the average heights of seedlings reached 2.10 cm.3. Embryo seedlings elongation cultureThe optimizing medium for embryo seedlings elongation culture was MS medium with GA 2.0 mg/L, the No.Of new leaves was 5.00, the No.Of new nodes was 2.67,and the average increments was 1.58 cm.4. Embryo seedlings rooting cultureThe optimizing medium for embryo seedlings rooting culture was 1/2 MS medium with NAA 2.00 mg/L, ratio of rooting is 80%, the average number of roots was 4.00, the average length of roots was 0.70cm. 5. Callus induction from young leaves in embryo seedlingsThe explants were young leaves in embryo seedlings. MS medium adding 6-BA 2.00mg/L and 2,4-D 0.30mg/L was Callus induced from young leaves in embryo seedlings, the induction rate was 66.10%, and the callus was yellow-green and close. Comparing to the culture under light, the culture under dark can form callus more efficiently, and the best time for the culture under dark was two weeks.6. Proliferation cultivation of callusHighly proliferation callus was obtained from the young leaves following cultivation on MS medium supplemented with BA 2.0 mg/L, 2,4-D 0.1 mg/L and NAA 0.05 mg/L. The proliferation rates was 72.41%. And callus differentiation was not accomplished.7. Tender stem initial cultureThe explants were tender stem from young embryo seedlings. The optimizing medium for tender stem culture was MS medium with 6-BA 2.00 mg/L and NAA 0.10 mg/L. The initiation rate could be up to 55.77%. And shoot proliferation was not accomplished.