Effects Of Sodium Selenite On Grass Carp Kidney Cells And Channel Catfish Ovary Cells

The effects of sodium selenite (Na₂SeO₃) on the morphology, growth and antioxidant capacity of channel catfish ovary cell (CCO) and grass carp kidney cell (CIK) were investigated. The effects of sodium selenite on channel catfish virus (CCV) and reovirus of grass carp (GCRV) were also investigated.The results showed the effects of Na₂SeO₃ on CCO were in dose and time dependent manners. Low doses of Na₂SeO₃ had little effect on CCO, and high doses of it (≥5μmol/L) inhibited the cell viability significantly, even induced cell death. The longer the incubation time was, the stronger the damage was. Na₂SeO₃ at high concentration (≥10μmol/L) induced CCO oxidative damage due to its oxidant properties. Significant decreases in activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were observed. The level of lipid peroxidatioin (the content of malondialchehyche-MDA) and the release of lactate dehydrogenase (LDH) were increased significantly. Na₂SeO₃ at the concentration of 10 nmol/L promoted the growth of CIK significantly. Higher doses of Na₂SeO₃ and longer incubation time inhibited the cell viability significantly. Na₂SeO₃ at high concentration (≥1 mmol/L) induced CIK oxidative damage due to its oxidant properties. CIK was more sensitive to Na₂SeO₃ then CCO. Na₂SeO₃ at concentration of 1μmol/L could protect CCO and CIK cells against viral infection and reduce cell damagement caused by CCV and GCRV.