The Epidemiological Investigation Of Streptococcus Suis Type2about Shihezi Reclamation

In order to understand the scale of pig farms Shihezi part Streptococcus suis type2infection status, type2of swine streptococcus disease serological surveys conducted, pathogen isolation and identification, biochemical identification, molecular identification, susceptibility testing, a small Experimental infection of mice and other research. For the prevention and control of disease provided technical support and basis.1Application of serological methods Shihezi14scale pig farms287sera were Streptococcus suis serotype specificity of the antibody testing. The results showed that:the positive rate was78.57%farms (11/14), seropositive pigs was41.11%(118/287), prevalence and infection are more serious.2pairs of53lung lesions were isolated streptococcus swine type2culture and biochemical identification, and type2suspected swine Streptococcus isolates detected by PCR molecular biology of Streptococcus suis type2cps2J, ef, mrp virulence factors. Results:44suspected bacteria, among them,21type2streptococcus swine, of which eight virulence factor cps2J,10virulence factor ef,3virulence factor cps2J/ef.3respectively, will carry cps2J, ef, cps2J/ef pig virulence factors in type2strains of Streptococcus THB and cultured for12to24hours. Press0.2mL,0.5mL,1mL dose infections were inoculated into mice intraperitoneally. Results:After48hours,1mL group containing bacteria virulence factors ef group one mouse died (1/3), containing bacteria virulence factors cps2J/ef group all mice died (3/3), containing virulence factors cps2J mice in the group were killed bacteria (0/9);0.5mL group containing bacteria virulence factors cps2J/ef group one mouse died (1/3), its vestiges of power factor bacilli group mice were killed;0.2mL group of virulent bacilli factor group mice were killed; All virulence factors injected bacteria group is not the death of mice, have shown spiritual malaise, feed and water decreased. Therefore, with cps2J/ef pig two virulence factors of pathogenic streptococci strong ef virulence factors including type2streptococcus swine certain pathogenic virulence factors including cps2J swine streptococcus type2no obvious pathogenicity. The results show that:with cps2J/ef virulence factors streptococcus swine type2Shihezi scale pig farms is a major pathogen.Mice were dissected discovery of death:lung and heart have different degrees of bleeding and bruising lesions, pleural and peritoneal exudate, easily solidified. And from the death rat disease compound isolated, cultured and identified the corresponding pathogen.4susceptibility test results show:SS2strains on the whole enrofloxacin, penicillin potassium, ceftiofur is highly sensitive to ciprofloxacin, kanamycin, ampicillin, florfenicol sensitive to moderately sensitive, for oxytetracycline, gentamicin low sensitive to sulfadiazine insensitive. Clinical medication can use enrofloxacin, penicillin potassium, ceftiofur high sensitizing drugs for treatment.