Studies On Tissue Culture Of A Special Aquicolous Vegetable Called Vallisneria Spinulosa Yan

This study was to explore the special aquatic vegetables Vallisneria barbed effective means of vegetative propagation of tissue culture in the process of disinfection methods, media types, explant selection, light temperature treatment, the choice of auxin and cytokinin etc. A comparative study. Buds sharp focus on the best procedures for tissue culture medium, the establishment of Vallisneria gill tissue culture system.The results showed that:1. Rhizome Vallisneria 6BArbed as the lateral bud explants, the choice of disinfection drugs showed that 70% alcohol to disinfect with 30s, and then increase 0.1% Mercuric chiorideand tween 20 (3～5 drops) disinfection of 12min, the best disinfectant;2. Vallisneria 6BArbed tip of the bud of the early generation of tissue culture, MS medium than the effect of N6 medium and the survival rate of growth is better, so choose as the 6BAsic medium MS; adding agar of about 7.5g, solid medium;3. Different explant organs, under the same conditions on the rhizome, leaf, bud tip for induction, shoot tip callus formed structure, the color green bias; bud tip the shortest number of days to start the 5d, followed by leaf 7d, the slowest start rhizome 15d; bud tip the lowest survival rate of the highest pollution rates were 77.5% and 20% complete and easy to cultivate plants; leaves and stem segments of the successful induction of callus induction rate is a low but the realization of the plants from the callus regeneration; therefore the best option explants lateral bud of the bud tip of rhizome;4. To bud tip as a test material, tissue culture conditions for the best illumination light shading in the normal two weeks after processing (1000LX) then normal light (1500LX) better; the best culture around temperature is 20℃;5. Cytokinin concentration is higher than the growth hormone to promote cell differentiation, in the interaction of 6BA and NAA, the bud tip early on behalf of the survival rate increased with the concentration 6BA lower. 6BA0.2mg /l + NAA1.0mg /l, the transfer rate and differentiation rate of the Green reached a maximum of 92.8% and 85.7%; 6BA and IBA interaction, IBA and 6BA changes in the concentration of its survival rate was less affected. At this point select MS +6 BA2.0mg /l + IBA1.0mg /l for the good, the survival rate and the maximum rate to achieve the Green 71.4% and 75%. That is, the best combination of primary culture is MS +6 BA0.2mg /l + NAA1.0mg /l, or MS +6 BA2.0mg / l + IBA1.0mg/l.6. The same as IBA or NAA conditions, 6BA to 2.0 mg / L, respectively, when the multiplication coefficient is lower than 1.0 mg / L at the time of the proliferation factor, and the MS +6 BA1.0mg / L + IBA0.5mg / L and MS +6 BA1.0mg / L + NAA0.1mg / L for the best deal with the level of the two. MS +6 BA1.0mg / L + NAA0. 1mg / L conditions, 30d, 60d, 90d, respectively, the complex multiples of proliferation than other to handle, to 1.60,2.22 and 4.25, that is, the proliferation of plants suitable for the best combination of medium to : MS +6 BA1.0mg / L + NAA0.1mg / L.7. MS +6 BA + NAA treatments, 6BA to 0, NAA is 0.1 mg /l, the highest rooting rate reached 51%, the average maximum number of root section 4.8. MS +6 BA + IBA treatments, the rooting rate of increase in IBA with the reduction in the 6BA for 0, IBA0.5mg /l at the maximum rooting rate reached 41 percent, the average root number reached 5.0. Different concentrations of IBA and NAA levels, root length of no more than 1.7cm, and an average root length 6BA as the concentration of both increases. 6BA the same concentration of NAA levels to deal with the rooting rate than the IBA deal with large number of small root. And with a gradual increase in the concentration of NAA is not conducive to root development. Rooting culture medium for the best MS +6 BA0.05mg /l + NAA0.1.