The Gene Structure And Expression Analysis Of PMADS9 In Petunia

Petunia PMADS9 gene is a member of the AGL15-like clade of MADS domain regulatory factors, and Arabidopsis AGL15 is considered to promote embryo development and repress the progression of a specific subset of age-related developmental program in the flowering plant. We cloned the genomic sequence (EU338501) of Petunia PMADS9 by PCR and by the southern hybridization we know there is only one copy in the genome. And compared the gene structure by comparative analysis of homologous genes, the results showed that both Petunia PMADS9 and Tomato SIMBPll had a long third exon and large first and second introns, which is different from other homologous genes. By using bioinformatics analysis, there is a exogenous sequence has been inserted between the second intron and third exon.A real-time PCR was performed to observe the expression profiling of PMADS9, and the results showed that PMADS9 preferentially expressed in anther and ovary and had low expression in other organs, which is slightly different from Arabidopsis AGL15. By using different chemical and physical treatment to the seedlings of Petunia, we found PMADS9 expression responded to 2,4-D and GA3, and increased in different environment stresses, this suggest it involved in plant stress resistance.