Newcastle Disease Virus Isolated From Inner Mongolia, Comparative Study Of HN Protein Gene Sequence

This study used four different regions of Inner Mongolia NDV isolates (NMHS, NMH2, NMH3, NMB), inoculated in 9 to 10 day old chicken embryo in value-added, proliferation was determined using HA HA titer after the virus and the total RNA extracted with reference to GenBank, published at home and abroad NDV HN protein gene sequence, using DNAstar 6.0 and primer premier 5 software, designed for the amplification of NDV HN gene-specific primers, RT-PCR method using amplified Inner four HN gene cDNA fragments isolated, so that the purified fragment was linked with PMD19-T vector, and it transformed into Escherichia coli cells, the white blue-white selection (plasmid), using PCR and restriction enzyme digestion are two ways to identify the recombinant plasmid, the plasmid containing the results of the bacteria were sent to Takara Bio Inc. HN gene sequencing. The sequencing results using DNAstar 6.0 software With GenBank, the HN NDV published nucleotide and amino acid sequences were analyzed and compared to construct phylogenetic trees to determine their genotype. The results showed that: The total length of NDV Inner Mongolia region of four strains isolated HN are 1716bp, encoding aa are 571; four strains isolated in Inner Mongolia nucleotide homology was 97.4%～98.8%, and the vaccine strains LaSota distant genetic relationship, differences in nucleotides and amino acids larger, while Jiangsu and Guangdong isolates were highly homologous, genetic distance, and the basic part of the same genes.