Investigation Of Scarab Beetle And Study On Identification And Application Of Sex Pheromone Of Dominant Species

Many insects attract opposite sex to mate by releasing chemical information compounds, those substances are called insect sex pheromones. Although scarab beetle species are the most important underground pests, the sex pheromone of scarab beetle was rarely reported. Dominant scarab beetle species of our nation′s peanut main producing area were selected as research target, through laboratory and field test, A preliminary study on identified the sex pheromone of Anomala corpulenta (Motschulsky) and sex pheromone of Holotrichia parallela (Motschulsky) application technology were completed. The results were as follows:1. The peanut fields of main producing area were investigated in Henan province, the result shows that soil-inhabiting pests consist of Scarabaeidae, Elateridae and Gryllotalpidae. As the main pest, the density of Scarabaeidae was 0.80～18.90 per centiare in July and 1.00～12.33 per centiare in September. Identified those pests, Holotrichia parallela (Motschulsky), Holotrichia oblita (Faldermann) and Anomala corpulenta (Motschulsky) were dominance pest species. The density of white grubs was influenced by soil types, culture mode, environment and measures of pest control.2. Abdominal glands were excised from Anomala corpulenta (Motschulsky) virgin females and placeed in a screw top vial containing solvent. "Y" tube olfactometer and electroantennogram were used to test the potential activity of crude extracts. These results demonstrated that hexane is the best solvent for extract the sex pheromone of Anomala corpulenta; coupled gas chromatograph-electroantennogram detection assay the crude extracts, a EAD active compound (rentention time = 26.17min) was revealed by using male antennae stimulated with female extracts. Finally, we utilized gas chromatography-mass spectrometry to identify the active compound, preliminary confirmed that the sex pheromone of Anomala corpulenta is 5-Tetradecyldihydro-2(3H)-furanone.3. Some factors about sex pheromone of Holotrichia parallelas (Motschulsky) for field application were systematically compared. The results showed that, the treatment that blends of L-isoleucine methyl ester and R-(-)-linalool at a dose of 100mg/septa in the ratios of 4:1 captured significantly more males than other treatments; Dichlorom as solvent, green rubber stopper septa and the traps made by myself hung 1.5m above the ground were three factors good for capture males.