Diversity Of Symbiotic Bacteria In Honeybee Alimentary Tract

Multiple symbionts were lived in alimentary tract of insect, which endow their insect host multiple biological functions. Varity of bacteria were also isolated from the hoenybee alimentary tract. But their biological function,distribution in the alimentary tract,the dynamics with the honeybee ages and the diversity in different honeybee were few invovled in past research. In this study, the morphology characteristics of bacteria and 16S rRNA gene sequence analysis technology were employeded to study the honeybee intestinal bacteriaspecies. In addition, The denaturing gradient gel electrophoresis (DGGE) were used to performed the honeybee intestinal bacterial diversity research. The results were as follows:1 By morphologic and 16S rRNA gene analysis, 24 strains of bacteria were identified from the honeybee of Apis mellifera and the Apis cerana. They were grouped into 10 groups. The enterobacteriaceae, accounted for 57.14% of all bacteria, is dominant flora. The Staphylococcus sp., is the second dominant flora, accounted for 29.2% of bacterias; the Bacillus sp. accounted for 12.5%.2 Cluster analysis of 16s rDNA DGGE of the bacteria from the honeybee of same age in one colony of Apis mellifera showed that the similarity of the bacteria in the same age of honeybee was between the 40%～70%, most of them were higher than 60%. This indicated that the highly similarity of bacteria community in the same age honeybee. Whereas,Cluster analysis of the bacterial communities from the honeybee with different age at same colony showed that most of their similarity among were less than 60%, the bacterial community structure was significantly different.3 Cluster analysis of the bacterial communities from samples of healty and suffered from chalk brood disease colony show that the bacteria communities is different. Most of their similarity was less than 60%.4 Cluster analysis of the bacterial communities of honeybees with different age from different colony showed that both the age and colony of Apis mellifera have a impact on bacteria community, their similarity was less than 60%, the bacterial communities structure among them was significantly different.