Development Of Microbial Fertilizer Using The Stroma Cusion Material From Non-pollution Pigsty

An aerobic fermentation test was conducted using the pig-pad as a medium from the non-pollution pigsty, infected seven types of biocontrol microorganisms,such as non-pathogenic Fusarium oxysporum, Paecilomyces lilacinus, non-pathogenic Pseudomonas aeruginosa, Condencate bacillus LPF-1, Brevibacillus brevis JK-2-GFP strain, non-pathogenic Ralstonia solanacearum and Bacillus cereus strain ANTI-8098A strain to study the fermenting technical conditions of the bio-fertilizer respectively. In the reseach, we had selected the composting samples as experimental materials to test the cultivation characteristics of the bacteria in the bio-fertilizer and the survival status of the biocontrol microorganisms through the morphology, gfp gene detection and 16srDAN molecular identification. The effect of inoculating microbes on the bio-fentilizer microbial communities was conducted using PLFAs (phospholipids fatty acids) method and diluting separation method. Simultaneously it was studied that the effect of inoculating types of microbes on the bio-fentilizer on the physical and chemical properties. Finally the the fermentation of Paecilomyces lilacinus was studied. The main results of this study showed as follows:1. after the Inoculation of the strains, the bio-fertilizer rapid rise in temperature, and compared with the control the treated groups by strains is higher in temperature ,among these biocontrol microorganisms Bacillus cereus strain ANTI-8098A compost fermentation temperature is most affected, meanwhile the quick-carbon amounts rapidly decrease. In addition, compared with the control , pH value in difference is not obvious.2.. The morphology, gfp gene detection and 16srDAN molecular identification showed non-pathogenic Fusarium oxysporum, non-pathogenic Pseudomonas aeruginosa, Brevibacillus brevis JK-2-GFP strain, non-pathogenic Ralstonia solanacearum and Bacillus cereus strain ANTI-8098A strain can survive in the bio-fertilizer, including the amounts of Brevibacillus brevis JK-2-GFP strain reached 6.23×107cfu/g in the fouth day.3. The adjunction of biocontrol microorganisms modified bio-fertilizer microbial community composition. the microbial communities in the bio-fertilizer are very abundant. Different types of micro-organisms in bio-fertilizer have different distribution. After the inoculation of the strains, the strains against bacteria and fungi a greater impact, while the actinomycetes less affected. 52 of PLFAs biomarkers were detected out by the MIDI microbial identify system,which constructed the finger printing of the microbial community in the stroma cushion in the non—pollution pigsty. PLFAs biomarkers distributions were significantly different among the different treatmeng, which could be divided into two types: completely distribution and incompletely distribution.4. Finally, this paper has studed the fermentation of Paecilomyces lilacinus which is processed into fertilizer drugs. Paecilomyces lilacinus is a biocontrol strain which can kill the plant nematode. The results showed that it can not survive in bio-fertilizer without adding foreign substances; when the quick-carbon source of sucrose is added the bio-fertilizer, Paecilomyces lilacinus rapid growth, and can rapidly reach 108cfu / g which standard the spore content of the of the microbial fertilizer.The results obtained in this study could be further use for fermentation technique and layed the theoretical basis on bio-fertilizer.