The Preliminary Proteomics Study On Interaction Between Rootstock And Scion Of Budgrafted Rubber Tree

Budding is the major planting material in the application of Hevea brasiliensis cultivation. It is known that there is a significant effect of rootstocks on the growth, yield and resistance of the budding trees. So it is very important to study the relationship between the rootstock and scion for the improvement of selection and application of rootstocks. In this study, the experimental materials, latex and bark were collected from the self-grafted and inter-grafted rubber tree with Haiken2(PB86×PR107) and Reyan 88-13(RRIM600×PiIB84), regenerated by anther wall somatic tissue culture, the some proteins involving the interaction between the rootstock and scion were separated and identified by proteomics research methods for further clarifying the mechanism of interaction between rootstock and scion in the aspect of proteomics. Results were summarized as follows:1. The latex proteins of rootstock and scion from the different experimental materials were extracted by TCA-acetone precipitation, two-dimensional gel electrophoresis(2-DE) was carried out to separate latex proteins, a comparative analysis of the pattern of latex proteins derived from the pattern analysis software, different protein spots were identified by MALDI-TOF MS.32 differentially expressed proteins were identified. These proteins include Os05g0105900, pre-mRNA splicing factor, methylenetetrahydro-folate dehydrogenase, putative, cell division cycle protein 48, ribophorin I family protein, ribosomal protein S8, Os12g0271600, transitional endoplasmic reticulum ATPase, hypothetical protein LOC_Os12g24270, light-harvesting chlorophyll-a/b protein of photosystemⅠ. Based on functional classification, they involved in transcription, translation, photosynthesis, cell division, protein synthesis, the latex synthetic and other biological processes.2. The bark proteins of rootstock and scion from the different experimental materials were extracted by TCA-acetone precipitation, two-dimensional gel electrophoresis(2-DE) was carried out to separate bark proteins, a comparative analysis of the pattern of bark proteins derived from the pattern analysis software, differential protein spots were identified by MALDI-TOF MS.52 differentially expressed proteins were identified. These proteins include photosystemⅡprotein 33kD, condensin complex component SMC1, Putative mudrA protein-maize transposon MuDR, classⅢperoxidase, maturase K, putative photoreceptor-interacting protein, heat shock factor, taxadiene synthase, theta class glutathione transferase GSTT1, calcineurin B-like protein 9, putative ATP synthase beta subunit, Oxygen-evolving enhancer protein 1, chloroplastic, phytochrome B, peroxidase, mitochondrial F1-ATPase beta subunit, phosphoenolpyruvate carboxylase, Ethylene receptor 2, pollen-specific profilin3, FLP1(FPF1-LIKE PROTEIN 1), arginase, cytochrome, ATP binding protein, putative. Based on functional classification, they participated in the latex synthesis, photosynthesis, transcription and translation, stress resistance, metabolism and other biological processes.3. Although the differentially expressed proteins isolated and identified from the bark and latex are different in the number and types, their functions mainly related to the transcription and translation, photosynthesis, the latex synthesis and other biological processes. Thus, we speculate that the interaction between rootstock and scion is existed in transcription and translation, photosynthesis and the latex synthesis at least.