Obtaintion And Identification Of Part Chinese Cabbage-Cabbage Disomic Alien Addition Lines

In order to creating Chinese cabbage-cabbage disomic alien addition lines,part Chinese cabbage-cabbage monosomic addition line was used, and its selfing F1 generation was tested by means of identification of cytology and SSR analysis,Three disomic chinese cabbage-cabbage chromosome alien addition lines were identified. Transmission rate of gametes and seeding set of Chinese cabbage-cabbage monosomic alien addition line were counted. This study provided basic material for creating alien substitution lines and translation lines of Chinese cabbage and cabbage, and helped to carry out Chinese cabbage genetic improvement, explore the relation between A and C genomes, study gene intactions, location and expression. The results were as followed:1.With Chinese cabbage(2n=2x=20) compared, seven monosomic aline addition lines seeding set by self were counted. Result indicate that monosomic aline addition lines seeding set by self were lower than Chinese cabbage. 6# Chinese cabbage-cabbage disomic alien addition lines seeding set was the highest,the rate was 65.48%,9# disomic Chinese cabbage-cabbage alien addition lines seeding set was the lowest,the rate was 14.40%。2.Reciprocal crosses were made between the part Chinese cabbage-cabbage monosomic aline addition lines(2n+1) and Chinese cabbage.The transmission rate of gametes the monosomic aline addition lines from alien addition lines were determined. Result indicate that the n+1 female gamete transmission rates of 2#,3#,4#,5#,6# Chinese cabbage-cabbage monosomic alien addition lines were respectively 25.64%,48.72%,28.95%,32.35% and 33.33%; The n+1 male gamete transmission rates were respectively 19.05%,28.57%,34.09%,19.51% and 29.79%。3. The F1 generation of three Chinese cabbage-cabbage monosomic aline addition lines selfing were indentified by taking account of the chromosome number in mitosis metaphase.The 2#,3# and 5# chinese cabbage-cabbage disomic alien addition lines were obtained.The rate is 10.09%4.PMC meiosis behavior of three Chinese cabbage-cabbage disomic alien addition lines showed that: the chromosome configurations at diakinesis were mainly showed 11II ,two chromosomes free move to cytoplasm at metaphaseⅠ, the chromosome configurations at anaphaseⅠwere mainly showed 11-11,it has many chromosome separation at MetaphaseⅡ,but chromosomes mainly distribute with 11/11/11/11.5.The field character of the Chinese cabbage-cabbage disomic alien addition lines, such as leaf surface character, leaf color, waxy of leaf,floral organs and so on, were similar to cabbage.The different cabbage chromosome caused different morphological features.6.The characters of pollen characteristics and seeding set were counted. The results showed that there was significant difference between three Chinese cabbage-cabbage disomic alien addition lines and Chinese cabbage. Significant positive correlation was pollen regularity,pollen viability and seeding set.7.The primes of BN72a and Ol10B01 were amplified on cabbage, allotriploid hybrids,5# Chinese cabbage-cabbage disomic alien addition lines and 5# Chinese cabbage-cabbage monosomic alien addition lines. The amplified result that 5# Chinese cabbage-cabbage disomic alien addition lines and 5# Chinese cabbage-cabbage monosomic alien addition lines were similar to cabbage,but Chinese-cabbage didn't have.It make out the alien chromosome of 5# Chinese cabbage-cabbage disomic alien addition lines corresponded with 07 linkage group of cabbage.