The Effects Of Salix Babylonica Polluted By Heavy Metals On The Physiological And Biochemical Responses In The Plagiodera Versicolora And Chaitophorus Salinigra Shinji

With the rapid development of city and modern industry,many environmental problems are induced, especially the heavy metal pollution is attracting more and more attention of increasing people. In this study, growth and development pritein content,free radical content, three protective enzymes (SOD,CAT and POD), detoxifcative enzymes(GST-s,AchE and CarE);heavy metal content of plagiodera versicolora and Chaitophorus salinigra Shinji fed on the leaves of Salix babylonica with various Pb,Cd,PbCd stress were measured respectively by laboratory rearing and spectrophotometric determination to reveal the changes of physiological and biochemical indexes in different insects after eating Salix babylonica polluted by different kinds of heavy metals stress, and provides the theory basis for further research on metal stress response mechanism of insects by heavy metal pollution. Main results are as follows:1.There are different influence trends on body lengths,head capsule widths,food Consumption,food utilization and increasing rate of body weight in plagiodera versicolora larvae.The normal growth and development of plagiodera versicolora was restrained significantly after Pb,Cd and PbCd stress.2.The resistance ability of adults is higher than plagiodera versicolora larvae; Protective enzymes were mainly affected in early stages (1st larvae,2nd larvae) of growth and development after different heavy metal stress, and detoxifying enzymes were mainly affected in the later stage, especially in pupa.3.The protective enzymes activity in Chaitophorus salinigra Shinji representd every eight days for a cycle, namely there are the same change trends between the 16-24d and the 4-12d.The Ache activity in Chaitophorus salinigra Shinji were significantly increased in the 24ds after Pb,Cd and PbCd complex stress, but not significantly in the early 20ds. The CarE activity of changes obviously in the prophase, and later did not significantly. GST-s transferase representd every four days for a cycle, namely there are the same change trends between the 12-16d,20-24d and the 4-8d. 4. The change trends of physiological and biochemical indexes in plagiodera versicolora and Chaitophorus salinigra Shinji eating willow leaves by the same mass fraction of Pb,Cd,PbCd stress were different slightly.And the influence of cadmium, lead compound stress on plagiodera versicolora and Chaitophorus salinigra Shinji was greater than the lead, cadmium single stress.5. The content of Pb in Chaitophorus salinigra Shinji was higher than plagiodera versicolora and the content of Cd was significantly lower than plagiodera versicolora after eating Salix babylonica polluted by the same mass fraction of heavy metals.