The Effect Of Boron Treatment Towards Differential Proteins Of The Leaves And Embryoes Of The Castanea

Chinquapin(Castanea henryi Rehd.& Wils.) is a kind of northern Fujian with distinctive fruit characteristics, there are a lot of economic value,. due to the pHenomenon of the empty studio exists in a long tine, It has affected the development of this industry at some extent. this experiment handled the samples through the boric acid solution .using the protein in two-dimensional electropHoresis, mass spectrometry identification and bioinformatics research methods to analyze leaves and immature embryos of protein in differences in order to reveal the normal growth and boron promoting plant growth,and improve fruit setting rate of molecular mechanism.1.Chinquapin(Castanea henryi Rehd.& Wils.)extracted by phenol extraction of protein in leaves and embryos and found that the majority of the protein concentrated on the pH5-8 range.2. Different proteomics reseach of leaves and immature embryos by Boric acid solution treatment was carried out Through establishing two-dimensional electrophoresis image analysis software system of leaves and immature embryos of Chinquapin(Castanea henryi Rehd.& Wils.) and image of proteins analysis software analyzing,The result showed that Boron treatment leaf total protein spots were more than the untreated leaves 88 points, most point of the increase concentrated in the acidic side; from molecular perspective, the increase in points, mostly in medium molecular weight range. Digging 13 different proteins whose abundance differences in the expression were more than twice identified by mass spectrometry for further successfully identified seven, divided into four categories, namely the relevant proteins involved in pHotosynthesis(RuBisCO Large Subunit-binding Protein Subunit alpHa,Ribulose 1,5-bispHospHate carboxylase), resistance proteins(Stress and pathogenesis-related Protein,Câ…¡small heak shock protein 1 ), unnamed proteins and unknown proteins. Unknown protein specific to untreated leaves, which may be closely related to B-deficiency stress; involved in pHotosynthesis, resistance protein and there is unknown protein-specific treatment leaves may be induced by the element boron and is closely related to the normal development of leaves.the untreated embryoes'spots were more than 137 points comparing treated , the increase in untreated embryos and protein spots, from the perspective of isoelectric point say, pI5-6 is within the largest increase; from molecular perspective, the most increase in protein is 66.2KDa range, in the following areas 35KDa protein spots has not increased but reduced. Make further mass spectrometry identified 16 of them dealing with differences in embryo two proteins belong to the resistance-associated protein(Putative glycine-rich RNA-binding protein GSTF3,pHi class glutathione transferase), may be stress related protein-B-dependent. Difference between untreated normal embryo protein 14, is divided into five categories, belonging to resistance-associated protein(Agglutinin isoform,small heat shoch protein classâ… ), energy metabolic-related proteins(DNA-binding protein-related,NADH dedydrogenase 1 alpHa subcomplex subunit 5), signal transduction class-associated protein(Hydrolase/protein serine/threonine pHospHatase,received Serine/thrconine protein kinase), cytoskeleton(Profiling-6,Profiling-1), and an unknown protein, these differences proteins interacting together in order to Embryonic resistance to boron deficiency caused by all the coercion, to ensure the normal development of embryos.3. Different proteomics research of different developing state of Chinquapin(Castanea henryi Rehd.& Wils.) was carried out.Using two-dimensional electropHoresis and protein pattern analysis software, obtained different developmental status of embryos differentially expressed proteins and found that the normal embryo proteins are more 170 protein spots than the empty seededness differentially expressed proteins from the increase in acid followed by reduction to pH7 ; from molecular point of view, the 66.2KDa is the largest increase in the protein region, the regional proportion of the total protein spots, two sets of little change. 13 differentially expressed proteins might be interested in digging further mass spectrometry successfully identified three points,the empty seededness embryo including 2, respectively, signal transduction(CyclopHilin) and resistance-associated protein(HSP19 classâ…¡), maybe, The CyclopHilin is involved in the death process of the embryoes'cell. The one in normal embryos is synthesis-associated protein(Acidic ribosomal protein P2), may be involved in synthesis of embryos required for normal growth and development-related proteins or enzymes.