| Acacia melanoxylon is Mimosaceae,the Acacia Mill,woody plants,originating in Australia in the 20th century,in the early 1990s was introducted to China,in Guangdong,Hainan planted good performance.Acacia melanoxylon have rapid growth,barren resistance,adaptability,and other characteristics of fine material.In recent years,large areas of planting started in Fujian,so emergenced seedlings shortage.This paper choses two Acacia melanoxylon fine clones stems FM25 and NO.4 as the basic materials to find fine clones stem,sterile system from the basic medium,environmental conditions of tissue culture,different organic additions,as well as the mechanism aspects of rooting and provide a basis theoretical and technology system for factory breeding.The main results were described as follows:1.From the rooting results of the two Acacia melanoxylon clone tissue culture, you can find auxins IBA and ABT-1 are more suitable to their rooting.IBA0.4mg/L-0.6mg / L,ABT-11.0 mg/L-2.0mg/L and AC0.1g/L together have a higher rooting rate.FM25 rooting rate is up to 98% and NO.4 is up to 80%.2.Chose the different MS for rooting ,we can finde 1/2MS is more suitable for the rooting of Acacia melanoxylon culture,markedly better than the MS,and 1/4MS.N element content is conducive to the improvement of tissue culture of Acacia melanoxylon seedling rooting,organic elements and Fe element have little effect.3.Rooting under different forms of medium found that solid - liquid mixed state is more suited to rooting culture medium,both media conducive to root absorption of the material, without affecting the root respiration,which will help the root elongation.4.Different sucrose concentrations on the rooting of tissue culture affected differently, Acacia melanoxylon two clones rooting culture need sucrose concentration 10g/L-20g/L.5.Breathability and support impact the root of tissue culture medium,this study found that agar as a supporting body of rooting rate is higher than the sand as a supporting body,plants died under Sand Culture serious.Permeability of the culture vessel is conducive to cultivating the plants roots.6.Study found that the rooting rate of plant with dark treatment is higher than an eight-day processing and dark treatment7.In this experiment,we can acknowledged that FM25 and NO.4have the best rooting rate and rooting amount under 2500lux.Rooting rate and root length under red light were higher than the blue and white, with an average root number performed better under red light.Rooting rate,root number and root length under 25℃perfact significantly better than the other temperature,the temperature of 22.5℃roots decreased significantly,indicaed low temperature is not conducive to the rooting culture of Acacia melanoxylon.8.The experiment show that medium supplemented with ree 10mg/L-20mg/L help rooting of Acacia melanoxylon culture,the effect of the different ree is not very obvious.Adding 1g /L of pancreatic protein frozen perfect well.Adding the banana and coconut mud Juice to Medium of the rooting is not good to the results,just to make plants more strong.9.In the process of Acacia melanoxylon rooting,the content of soluble sugar changed with the culture time,in the process of rooting,the soluble sugar in the body of FM25 first decreased and then increased. soluble sugar content of For NO.4 rised after the first decreased,but decreased at last,which is relate to the low rooting rate .10.Chlorophyll fluorescence parameters of different clones perfect difference in the process of rooting,the changes of Fo are different increased at first and then reduced and increased at last.The fluorescence parameters Fm,Fv / Fm,Fv / Fo changes perfect different,which is ralated to rooting.
|
PDF Full Text Request