Study On Propagation, Making Strong And Rooting Of Adventitious Buds Of Acacia Cincinnata

Acacia cincinnata widely distributes along the coast of Queesland Australia, Southern Papua New Guinea, Eastern Indonesia. It was introduced to our country in the 20th century 70s. In this study, in order to meet the needs of factory production, we studied on prolif-eration of Acacia cincinnata buds, strengthing and rooting. The main results were describedas follows:1. proliferation of 2nd and 3rd Acacia cincinnata buds. According to single factor experimental design and orthogonal design methods, to study these factors: seal materials, plant growth regulators, tissue size, cultivation method, light intensity, subculture period, affect on proliferation. The optimum hormone combinations for 3rd buds proliferation is: MS+ 6-BA0.8mg.L^-1+IAB0.01mg.L^-1+GA30.3 mg.L^-1. The condition is liquid culture. The optimum hormone combinations for 2nd buds proliferation is: MS+6-BA1.0 mg.L^-1 +IBA0.01mg.L-1+GA30.3mg.L^-1. The conditon is the same to 3rd buds. The seal materials are both breathable membrane and the light intensity is 200 umol.m-2.s^-1. Proliferation of a tissue with 5-7buds is better. The hightest proliferation coefficient of two kinds of source were 4.094 and 3.597.2. 3rd and 2nd of Acacia cincinnata strengthening before rooting. Considering the effect of tissue size, plant growth regulators, NaH₂PO₄ and AC on 3rd and 2nd of Acacia cincinnata strengthening, Random combination of experimental design and orthogonal experimental design were used. The optimum hormone combinations for 3rd Acacia cincinnata strengthening is MS+6-BA0.5 mg.L^-1+IBA0.2mg.L^-1+NaH₂PO₄150mg.L^-1+AC0.3mg.L^-1;Theoptimum hormone combinations for 2nd Acacia cincinnata strengthening is: MS+6-BA0.4 mg.L^-1+IBA0.2 mg.L^-1+NaH₂PO₄250 mg.L^-1+AC0.3 mg.L^-1. The tissue size with 3-4 buds was better. The stems of two source were both strong and lignification.3. According to the single factor experimental design, orthogonal experimental design and the random combination of experimental design, considering impact of plant growth regulators, basic medium, sucrose, dipping method and two-step root method on rooting of 2nd and 3rd Acacia cincinnata.The optimum rooting method for the two source of Acacia cincinnata was dipping method and two-step root method. If use dipping method, the best way for the 3rd Acacia cincinnata was: frist dip in 1500ppm IBA solution for 4s and then inoculate the media without any plant growth regulator. The rate of rooting was 100%, and the average of root number was 7.0, and the average of root length was 3.2cm. The best rooting method for 2nd Acacia continma was: frist dip in 1500ppm IBA solution for 4s and then inoculate the media without any plant growth regulator. The rate of rooting was 100%, and the average of root number was 6.6, and the average of root length was 4.3cm. In two-step root method, considering root time, root rate, the average of root number and the average of root length, the rooting method for 3rd Acacia cincinnata was: fristly cultured 4 days on the media of 1/2MS+IBA5.0mg.L^-1 with 20g.L^-1 sucrose, and then inoculate the media without any plant growth regulator. The best light intensity was 200umol.m-2.s^-1. In this way, root rate was 100%, and the average of root number was 4.9, and the average of root length was 4.0cm. The best induction method for 2nd Acacia cincinnata was: cultured 7 days on 1/2MS+IBA4.0mg.L^-1 media with 20g.L^-1 sucrose in dark, and then inoculated the media without any plant grownth regulatorc with 200umol.m-2.s^-1 light intensity. The rate of rooting was 100% and the average of root number was 7.6 and the averge of root length was 5.8cm.