Isolation And Identification Of Mycoplasma Gallisepticum Isolated From Tibet

Mycoplasma gallisepticum(MG)is a primary pathogen ceausing chronic respiratory disease of chicken, which is characterized by coughing, nasal discharge, rattling and inflammation of the saccus pneumaticus. MG infection is easily contagious and often lead to the disease of chicken flock, but the mortality is not very high, for it is often the subclinical infection chickens that lead to egg production of layer dropped and hatchability reduced, which causes serious losses in the poultry industry. The current control of the disease proceeds from two aspects: first, to do well the vaccine that from economic or effect on prevention of infection is the best choice to control MG. The second is to improve health services and choice of work, good breeding environment for disease to prevent the most fundamental conditions, only by doing daily disinfection, and cleaning work to the source from the root and disease prevention.Isolation and identification were carried out for the suspicious infected chickens which came from different poultry farms in the area of Linzhi in Tibet. That was the purpose of this study. After being soaked for 24h in 25% solution of penicillin, 100μl culture were taken out and added to improved Hartleys medium with thallium acetate to amplify the bacteria. After 2 or 3 days, 3 isolates of the colony was observed with the appearance of fried egg.There were small globular short rhabditiform and irregular blue bacteria from the test under microscope by wright staining.Therefore the isolates were initially diagnosed as mycoplasma. The biochemical and serological identification, can determine which two to Mycoplasma gallisepticum.Based on the conservative sequence of Mycoplasma gallisepticum 16SrRNA partial sequence of the primer design with Primer premier5.0 software design of Mycoplasma gallisepticum isolates from a pair of primers. The purpose of PCR amplified 577bp fragment. Strain Tibet and Chongqing isolates by amplified fragment sequences were compared. Results 2 of Tibet and Chongqing strains isolated fragment homology were 96.7% and 96.8%. We used the method of SDS-PAGE of Mycoplasma gallisepticum isolates from Chongqing and Tibet to study the structure of protein. Then found two areas of structural proteins of Mycoplasma gallisepticum differences may be related to widespread use of antibiotics. Widespread use of antibiotics may lead to expression of Mycoplasma gallisepticum structure changes. Isolates the two regions of drug sensitivity results showed that isolates of Tibet is better than Chongqing. The reasons for this difference may be related to different regions and farms and also the different antibiotics used.