Studies On Olive Embryo And Stem In Vitro

In this study, olive (Olea europaea L.) embryo and stems were used as materials to establish an in vitro culture system. The main results were as follows.1.'Frantoio' were inoculated in March,April,May and June, explants isolated in April and May resulted in the lowest browning rate,23.4%, the other time (in March and June) were inoculated with the browning sampling rate of 45%,33.6%.2. Sterilization effects were different in different parts of the stems. Performance for the top section, second top section.â…¢andâ…£of the contamination rate decreased, the section III and section IV were the best, And 18min to the effect of disinfection best.3. White was the suitable basic medium with high sprouting rate, while MS was suitable for callus induction.4. The embryo culture was less strict in the requirement for hormones in the medium in the primary culture. However, proliferation was cultivar dependent and'Tanche'showed the best proliferation performance in the medium White+6-BA2.0mg.L-1+NAA0.3 mg.L-1+GA32.0mg.L-1.5. The stem culture was more cultivar dependent, the hightest was 16.7%. Of the cultivars used in the study,'Frantoio' was the most responsive whose callus induction rate and sprouting rate were 58.3% and 6.7%, respectively.6. The best rooting medium was 1/2 MS+NAA2.0mg.L-1+6-BA0.3mg.L-1, in which rooting rate could reach 86.7%.7. In vitro plantlets were put inside the room at the normal temperature for 7 days, then were put outside for hardening.30 days later, bottles were opened for 7 days. Then transplantation was followed, resulting in a survival rate of 11.9%.