Study On Candidate Genes Of A Major QTL For Salt-tolerance By Genetical Genomics In Soybean(Glycine Max)

Soybean (Gycine max L.) is one of important economic crops of China. Drought and salinization are the two major causes leads to cultivable land and yield decrease. Modern biotechnology is an effective way to improve the salt tolerance of soybean which is a promising approach to solve the problem of salinization.According to the fine mapping information of soybean salt tolerance main QTL from co-operation, salt tolerant main QTL was mapped in the interval of 39.69Mbp-44.06Mbp on the third chromosome by genetical genomics analysis. About 746 ORFs were predicted from this fragment by FGENESH on Softberry. All ORFs'function was predicted through BLAST analysis. And about 19 ORFs were identified which may contribute to soybean salt tolerance trait. Hefeng 39 seedlings were treated with 150mM NaCl, and all 19 candidate genes'time-spatial expression patterns were analyzed by real time quantitative PCR. After salt stress, gene expression of ORF4-442 was up-regulated significantly both in roots and leaves; meanwhile, ORF7-316, ORF17-641 and ORF19-737 were only up-regulated in roots and down-regulated in leaf significantly.Full length cDNA of 4-442,7-316,17-641,19-737 will be cloned, and transformed into Arabidopsis and rice by Agrobacterium-mediated transformation to further verify these four genes'function in vivo in following work.In addition, Gm179, one former identified salt tolerance candidate gene in our lab, was cloned, sequenced and transgenic constructor was constructed, which has laid a solid foundation for further functional analysis of Gm179 in vivo.