Study On Enzyme Fodders, Feces Mediums And β-Molting Hormone Of Yellow Mealworm (Tenebrio Molitor L.)

Tenebrio molitor L. belong to Tenebrio of Tenebrionidae in Coleoptera. They are not only large-scale storage pests and important research materials, but also the extremely potential resources. This test mainly studied on the influence of Tenebrio molitor's growth from Cellulose, Hemicellulase and Xylanase, the influence of edible fungi's growth from Tenebrio molitor's feces medium as well as the methods and conditions of P-molting hormone extraction in Tenebrio molitor.1.Preliminary results from Tenebrio molitor's feeding of different Enzyme fodders(1) After feeding 30～40-day-old Tenebrio molitor by mixing the wheat bran and rice chaff, Alternanthera philoxeroides, corn stem, Trifolium L., rice stem, wheat bran in the ratio of 1:1, the result was obtained:the rice chaff could be the best choice on replacing wheat bran as feedstuff. After feeding for 30 days, the average fresh weighted 0.0246 g/larvae, the average fresh increasing by 0.0188 g/larvae, dry matter content for 39.98%, AD for 63.55%, ECD for 28.20%, and ECI for 17.93%.(2) After feeding 40～50-day-old Tenebrio molitor by mixing the wheat bran and rice chaff in the quality ratio of 1:4,2:3,1:1,3:2 and 4:1, the result was obtained:the best choice is mixing the wheat bran and rice chaff in the ratio of 2:3 as feedstuff instead of the whole wheat fodder. After feeding for 30 days, the average fresh weighted 0.04915g/larvae, the average fresh quality increasing by 0.03329g/larvae, AD for 62.34%, ECD for 29.74%, and ECI for 19.50%.(3) Using the two methods by pH test strips and pH-meter to determinate the pH value of Tenebrio molitor's intestines, the results were 4.3694 and 4.412 respectively. So the conclution is that the pH value of intestinal environment in Tenebrio molitor is about 4.4. (4) Using the Enzyme fodders, which were joined with Cellulose, Hemicellulase and Xylanase respectively, to feed 50～60-day-old Tenebrio molitor, the conclution were as follows:①The 0.8% cellulose fodders could promote Tenebrio molitor's growth obviously After feeding for 30 days, the average fresh weighted 0.07015g/larvae, the average fresh quality increasing by 0.05549g/larvae, AD for 64.04%, ECD for 31:41%, and ECI for 26.72%.②The 1.0% Hemicellulase fodders could promote Tenebrio molitor's growth obviously. After feeding for 30 days, the average fresh weighted 0.06724g/larvae, the average fresh quality increasing by 0.05251g/larvae, AD for 61.60%, ECD for 44.78%, and ECI for 26.69%.③The 0.8% Xylanase fodders could promote Tenebrio molitor's growth obviously. After feeding for 30 days, the average fresh weighted 0.06595g/larvae, the average fresh quality increasing by 0.05133g/larvae, AD for 63.24%, ECD for 37.88%, and ECI for 23.95%.2. The influence of edible fungi's growth from Tenebrio molitor's feces mediums(1) The optimal preparation method of Tenebrio molitor's feces leaching solution through the test is as follows:leaching 16h on the condition of 70 degrees after mixing the feces and water by the ratio of 25:100.(2) Cultivating Pleurotus ostreatus(Fr.)Kummer, FlammuLina velatiper(Fr.) Sing, Ganoderma lucidum (W.curt.:Fr.) Karst and Lentinus edodes (Berk.)Sing by use of solid and liquid mediums from Tenebrio molitof s feces leaching solution, the results show that:the hypha growth of the four mentioned mushrooms was the best in the flat mediums with Tenebrio molitor's feces leaching solution respectively being 25%,20%,25%,20%. In the liquid medium that used Tenebrio molitor's feces leaching solution as nitrogen source, the hypha growth of FlammuLina velatiper(Fr.) Sing, Ganoderma lucidum (W.curt.:Fr.) Karst and Lentinus edodes (Berk.)Sing was optimal with Tenebrio molitor's feces leaching solution being 20%; While the hypha growth of Pleurotus ostreatus(Fr.)Kummer was optimal with 25%.(3) The result of using Tenebrio molitor's feces to cultivate and product edible fungis shows that:the Tenebrio molitor's feces could cultivate Lentinus edodes (Berk.)Sing, Pleurotus ostreatus(Fr.)Kummer, Flammulina velatiper(Fr.) Sing, Agrocybecha Sing Hnang, Copyinds comatus(MUⅡ.Fr) Gray and Ganoderma lucidum(W.curt.:Fr.) Karst; The content of feces is higher, the Flammulina velatiper (Fr.) Sing hypha growth faster; The production of Flammulina velatiper (Fr.) Sing and Pleurotus ostreatus(Fr.)Kummer could increase with the Tenebrio molitor's feces.3.Preliminary studies on the methods of P-molting hormone extraction in Tenebrio molitorThis test used analysis method of Box-Behnken to analysis the effect on the extraction rate of P-molting hormone in Tenebrio molitor from interactions between time and temperature, and establish regression model table. The optimal Leaching condition was obtained:leaching 3 hours with ethanol on the condition of 80 degrees, with the extraction rate being 0.0563%. Besides, this test also obtained the optimal leaching ratio between material and liquid is 30:100, with the extraction rate being 0.0848%.