Fish Resources And ISSR Of Genetic Diversity Of Four Major Chinese Carps In Lower Reaches Of The Xiu River

Xiu River is located in Jiangxi Province, northwestern mountainous area of Jiangxi, one of five major rivers, originating from the Tonggu Country Gaoqiao township Yejiashan, located in east longitude 114°31′, north latitude 29°01′. Estuary for the town of Wucheng Yongxiu County, located in east longitude 116°01′, latitude 29°12′. The main river length 419km,drainage area is 14797km2, accounting for 8.9% of the total area of the Jiangxi province.This article base on river fisherises,four major Chinese carps and spawning grounds in Xiu River.and designed ISSR molecular markers to reveal the genetic structure of four major Chinese carps.For the propose of protection four major Chinese carps provide the scientific basis.The major findings are as follows:From April to August in 2009, we collected the number of four major Chinese carps in Yongxiu is 178, which included 57 black carps, accounting for the pecent of the total number is 32.02%; grass carp 78, accounting for the pecent of the total number is 43.82%; silver carp 17, accounting for the pecent of the total number is 9.55%; bighead carp 26, accounting for the pecent of the total number is 14.61%. four major Chinese carps accounted for about 1.68% of the total catch.From June to August in 2008,we collected the four major Chinese carps juveniles with the scam (mesh of 0.3×0.3cm) in Wucheng is 358. Among them, the number of black carp is 248, accounting for 69.2% of the total amount; grass carp followed, there are 57, accounting for 15.9%; followed by silver carp, there are 45, accounting for 12.5%; bighead carp at least, only 8, accounting for 2.2%.Compared with the past, the resource of the four major Chinese carps in Xiu River is decline; four major Chinese carps between the number of significant were changed; a younger age, small-scale phenomenon is relatively serious.The colonial genetic variations in four major Chinese carps were analyzed with ISSR, with 17 documented primer pairs Xiu River four ISSR amplification of four major Chinese carps, balck carp have six primers amplified polymorphic bands were detected 58 sites,of which 31 polymorphic loci, the proportion of polymorphic loci for 53.45%; grass carp has eight primers amplified polymorphic bands were detected in 75 sites, of which polymorphic loci 32, the proportion of polymorphic loci for 42.64%; silver carp had six primers amplified polymorphic bands were detected in 49 sites, of which there are 25 polymorphic loci, the proportion of polymorphic loci 51.04%; bighead carp 5 primers can be amplified polymorphic bands were detected out of 37 sites, of which 18 polymorphic loci, polymorphic loci compared to 48.64%. The results analyzed by software PopGen32 indicated that:the colonial genetic diversity in black carp in Xiu River (He=0.3561, Ho=0.5202); the colonial genetic diversity in grass carp (He=0.3561, Ho=0.5202); the colonial genetic diversity in silver carp (He=0.1524, Ho=0.2275); the colonial genetic diversity in bighead carp (He=0.2091, Ho=0.2999); diversity levels are high, there is greater genetic variability.