Studies On The Molecular Markers Linked To Yellow Characters And The Protein Changes Of The Yellow Leaf Color Mutant In Tomato

Leaf color variation is one phenomenon of the biological phenomena, so far many types of natural pigment mutants have been found in wheat, corn, barley, bean and rice and other crops. In recent years, The use of leaf color mutants were more concerned about value. In basic research, the leaf color mutants are an ideal material for the physiological metabolism processes, which included the plant photosynthesis, photo-morphogenesis, and hormone physiology and resistance mechanism. At the same time, the mutant genes can be used to identify and analyze the functional genes, and genetic interaction. In the work of breeding, color variation can be used as markers in seedling to identify the purity of hybrid to simplify breeding. Some leaf color mutants have special good characters which can provide excellent germplasm resources for crop breeding. So it has greater theoretical and practical value in agricultural production.In this study, using spontaneous mutation 06883 as the materials, using SSR markers and SDS-PAGE analysis of total leaf protein technology to promote understanding of the theory of the mutant to speed up the application of the mutant. The main results of this study are as following:1. There is no significant difference between mutant and normal plants in the growth potential. Pigment content in mutant leaves is lower than normal plants; it was the lack of total chlorophyll mutation.2. The character of the yellow leaf color was controlled by one recessive gene.3. Three SSR markers linked to the leaf color mutant gene were identified in the study, LEaat006, Tom196-197 and LEtat002, and the mutant gene genetic distance of the chain 8.9, 16.3and 18.7cM. Tom196-197 mark which is located on chromosome 11, therefore, we believe that the mutant gene was located on chromosome 11, and named the gene yl, identified the mutation as a new tomato leaf color mutant.4. For comparison, we found that the proteins in mutant leaves were not significant different from the control, while the protein 89KD, 65KD and 31KD were deficiency in yellow leaves, and 94KD, 70KD and 49KD protein was reduced compared to the green leaves, the absence and the reduce these proteins may associated with the reasons of the leaves turned yellow.