| Mentha spicata L, also known as green mint, are important economic crops. Spearmint originated in Europe, were introduced from the U.S. in 1980's. In recent years, spearmint acreage was increased widely. Spearmint oil is widely used in the food, and as well in medicine for the treatment of detoxification, inflammatory, antalgesic and haemostatic. Some researchers studied its antibacterial effect and found its antibacterial properties, but nobody reported its antiviral properties.Research on Antibacterial effect of Spearmint.The antibacterial function of Mentha spicata Linn extracts on 4 animal bacteria derived animals, Escherichia coli, Salmonella, Enterococcus, Staphylococci in vitro were studied by the agar Cup-plate method and the tube double dilution method to determine their Minimum inhibitory concentrations(MIC). The results showed that, the aqueous extract, the alcohol extract and the volatile oil of Mentha spicata L. can inhibit their reproduction especially.for Staphylococci. The MIC of aqueous extractâ… (sinking with alcohol) is 31.25mg/ml. The MIC of alcoholic extraction III is 62.5mg/ml. The MIC of volatile oil V is 2.32mg/ml. The MIC of volatile oil VI is 2.31mg/ml. Mentha spicata L. shows anti-bacterial effects on the experimental strains in vitro. Each kind of Mentha spicata extracts has distinctive inhibitory effect to different bacteria.Antiviral Research of Spearmint.The study explored anti-porcine parvovirus(PPV) effect of Mentha spicata Linn extraction in vitro. Antivirus effects of Mentha spicata Linn extraction on PPV were assayed by MTT assay and CPE, and the data were statistically analyzed. The study was tried in three different ways (extraction was administrated before infection, administration upon infection, administration after infection)cells infected with PPV established in vitro infection model. The results showed that, Spearmint oil had significant anti-porcine parvovirus effect in all three administration ways. The median inhibiting concentration(IC50) was 0.008mg/ml,0.0019mg/ml and 0.0036mg/ml respectively. the treatment index(TI) was 25.88,108.95,57.5 respectively. The aqueous extract and the rough extract had anti-PPV effect in two ways (administration upon infection, administration after infection). The IC50 was 0.034mg/ml and 0.356mg/ml respectively as the drug was added after it mixed with virus 2h. The therapeutic index(TI) was 79.18,8.37 respectively. The TC50 was 0.043mg/ml,0.063mg/ml when the drug was added 2h after infection, The TI was 62.60,47.27 respectively. We can conclude that Spearmint oil has the direct deactivation, blocking and preventive effect. The aqueous extract and the rough extract have the direct deactivation and blocking effect. Their preventive effect is not evident.A method to detect PPV by means of SYBR Green I real-time fluorescence quantitative PCR was established through this study, and applied to determine proliferation of PPV in the PK-15 cells, and according to it to determine DNA content change of PPV after treated by Mentha spicata Linn extracts. The results showed that the proper law of proliferation of virus is still present after drug treatment, but all stages are inhibited by the drug, with relatively fewer copies. A substantial reduction of viral DNA content of treatment groups in cell layer are less than 2 to 4 orders of magnitude compared with the virus control group, while the viral DNA content of treatment groups in the supernatant are less than 3 to7 orders of magnitude compared with the virus control group. Virus replication in the PK-15 cells is inhibited effectively, thus inhibiting the production of cytopathic. |
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