Straw Cellulase Ferment And Initial Search Of Product In Feeding Sika

100~ 200 hundreds billion ton botanical organic material were formed all over the world every, half of which are cellulose. The number of crops residue such as straw formed in agriculture production exceed 700 million ton. Meanwhile, there are millions of cellulosic waste materials in industrial production. But they aren't fully utilized as well as pollute environment. Therefore, governments and scientists always devote to researching and developing the abundant nature resource. But so far the utilization of straw hasn't reached to satisfying level. The main reason is lack of high activity fungus to absolutely degrade nature straw cellulose. Cellulose microorganism is utilized to degrade waste material. This method not only reduces pollution but also saves energy. Utilization of cellulose possesses profound and far-reaching siginificance. From this, people can get useful material from city cellulosic waste material. So far, cellulase has extensively been used on the field of industry, agriculture, animal stock husbandry and medical science. And this application has obtained preliminary results, especially in forage cellulase aspect. Although our country has researched cellulase for a long time, cellulase activity is low and production cost is expensive, so that cellulase application is limited. For this reason, screening and cultivating high activity cellulase is one of the important tasks in the field of enzyme preparation.Our country is a big agriculture country, straw resource is abundant, annual output reach to hundreds of million. But the use ratio of crop straw is still quite low in hometown. At present, straw is mainly used to fertilize fermentation. But this method can't solve synthetical utilization of a great deal of straw. Corn straw is crops residue which full of cellulose. It is important to convert cellulosic waste materials into simple carbohydrate or protein. Because it can solve environmental pollution as well as alleviate grain crisis. Consequently, isolating and cultivating high output cellulose decomposition fungus is a significant task. It is a development direction of present feed industry to produce fermented feed with cellulose degrading bacteria, which can hydrolyze cellulose into monosaccharides and increase the protein content in treated feed.The study used the liaofeng-2 mealie stalk as raw material which was treated by alkali, collected fungus from soil which full of microorganism such as hillside, firewood heap, and vegetable plot. We separated and screened out one fungus, it could produce the cellulase with a high rate and property is steady. By a principial identify with the biology, the fungus was Trichoderma viride. The experiment adopts liquid ferment, use cellulase ferment straw. By determining CMC enzyme activity, FPA enzyme activity, content of rough filter and protein, we could define optimal condition which this fungus product enzyme.The liquid fermentation result indicated that the total amount was 15 g/ L of the straw powder and the bran with 3:1 of comparison admixture as the best carbon source, the 1.7 g/ L ammonium sulfate mixes with the 0.5 g/ L peptone as the best nitrogen source in the experiment, the optimal inoculated amount was 3%, the most suitable pH was 6.0 for cellulase, the most suitable temperature was 30℃, the most suitable cultural time was 6 days. In addition, the metal ion, vitamin, active reagent of the surface and so on were important factors which affected the enzyme activity. In the optimized cultivate conditional experiment, the metal ion Na+ had a promotive function to the decomposition of cellulose and enzyme yield of the germ, but Cu2+ repressed obviously for the cellulose enzymy activity and the decomposition of the celluloses. Moreover, the vitamin B2 didn't increase enzyme activity, but improve decomposition rate of cellulose, increase the content of rough protein; the vitamin C had a promotive action to enzyme activity and decomposition rate of cellulose.0.2% Tween80 could strengthened the enzyme activity and production ratio.In addition, cellulase is an induced enzyme, cellulose decomposition require different microorganisms. In this trial, Trichoderma viride and Penicillium or yeast were co-cultured, cellulase activity and decomposition of cellulose both increased, the content of rough protein also improved. Among them, the mixture effect of Penicillium and Trichoderma is best. This suggested that the Penicillium and the Trichoderma had a stronger action to conjunction with each other, and could add mutually, thus promoted the decomposition of cellulose material.In this trial, Trichoderma and Penicillium were mixed to prepare for seed liquid. We adopted batch fermentation in 10 liter automatically controlled fermentation jar. Initial condition is stirred speed 800 r/min, temperature 30℃, cultivated time 6 days, CMC enzyme activity reached 4.6955IU/mL,FPA enzyme activity reached 1.4175 IU/mL,the content of rough cellulose was 11.01%,the content of rough protein was 20.66%. The result is ideal.We used corn straw sprayed cellulase liquid and residue of culture medium as rough feed. Raising experiment suggested adult male sika equally increase body weigh 29.56kg, daily increased body weigh was 492.71g, produced pilose antler raised to 1.44kg, rate of increase was 10.77%.In a word, this experimental result possesses directed function for cellulose in liquid fermentation product; it provides basis for development of straw, and establish foundation for herbivorous animal feed.