The Study On Mutagenic Effects Of Space Condition In Catharanthus Roseus And Mutant Selection

Catharanthus roseus is a kind of ornamental plant, but also is an important medicinal plant. Using C.roseus seeds space-mutated by recoverable satellite "Shijian-8" as material, and comparing with the same period of stay ground of the C.roseus seeds, to study on vegetative growth, reproductive growth, biomass and alkaloids of Catharanthus roseus by mutagenesis and to explore the space conditions on the mutagenic effects on C.roseus, and to provide materials for cultivating new varieties of C.roseus.There was significant difference in the content of vindoline, catharanthine and vinblastine which in different organs of C.roseus and the level contents of three alkaloids in leaves were highter, the higher contents of vindoline and catharanthine were in early August and September and that of vinblastine in the late of October. At the aspects of detecting time and position, we could detect the leaves as object at the last of October if the plant was used for high production of vinblastine, and at the early of September if the plant was used for high production vindoline and catharanthine. Three alkaloids as both targets we could choose the early September to detect at the fruits period of C.roseus.Space complex factors on the phenotype, biomass and alkaloid metabolism in both influence and mutagenic effect was remarkable. SP1 progeny had more phenotypic variation, and variation effects for the two-way. In general, growth potential and reproduction ability and the level of alkaloid content increased, and the observed changes in the scope of targets to increase the coefficient of variation increases. After three rounds selection aiming at the content of vinblastine, the content of dry weight of vinblastine was above 300ug/g in the leaves of four strains from SP3 generation we gathered which was more than three times of the control group, and the coefficient of variation in this strain was lower than 15%. Additionally, we had selected the plant with integrated strigose from plant 88. In this type of plant, the contents of three alkaloids had no significant difference; however, it had potential utility in cold hardiness.Compared and analyzed FTIR spectra of the leaves of C.roseus with space mutation and that in ground, both the front shapes were similar and the peak positions were consistent. Individual peaks were moving, but the induced group of the main absorption peaks intensity different degrees of enhancement, which indicated that the main components of mutation types were no obvious changed, but a variety of organic ingredients contents increased. Principal component analysis indicated the regression equation with correlation coefficient was 0.86 and coefficient of determination was 0.74, and 1047 cm-1 wave number of peak intensity,2926 cm-1 wave number of peak intensity,2894 cm-1 wave number of the peak area were better correlated with the content of vinblastine. The path analysis of phenotype and the contents of three alkaloids of C.roseus indicate the numbers of leaf scars marked a major influence on the content of vindoline and was a very significant negative correlation and path coefficient was-0.741. Catharanthine content and internode length showed significant positive correlation and path coefficient was 0.798. Vinblastine content only with below-branch height had significant negative correlation. At the aspect of phenotype selection which was highly relative to objective and active material, we could select the phenotype with less leaf scars if we targeted at vindoline content, while we could select the phenotype with large internode length if we targeted at catharanthine content, also we should select the individuals with lower below-branch height if we targeted at vinblastine content.