Effects Of Chlorogenic Acid On Production Performance And Antioxidant Function In Trionyx Sinensis

This study focused on the effect of different doses of chlorogenic acid on the production performance and antioxidant functionin in trionyx sinensis.80 healthy trionyx sinensises with an average weight of (140.78±24.18) g were selected, and randomly divided into 5 groups (A～E group) with 4 replicates in each group and 4 turtles in each replicate.0,100,200,400 and 800 mg/kg of chlorogenic acid were added in the diets of 5 groups respectively. The trial lasted for 60 days. The results showed that.(1) Chlorogenic acid had good effect on the growth of trionyx sinensis. Compared with control group,400mg/kg chlorogenic acid increased weight gain ratio 129.23% (P<0.05)(2) There were no differences in calipash ratio,intestine ratio,body fat ratio,muscle moisture,and muscle crude lipid among five groups (P>0.05)(3) Chlorogenic acid could promote intestinal growth of trionyx sinensis. Compared with control group,each chlorogenic acid group increased the height of intestinal villus in trionyx sinensis 25.52%(PB<0.01),60.35% (PC<0.01),52.30%(PD<0.01),50.95% (PE<0.01)(4) Chlorogenic acid could increase the content of muscle crude protein,muscle and skirt collagen,and reduce the content of muscle cholesterol in trionyx sinensis. Compared with control group,400mg/kg chlorogenic acid enhanced content of muscle crude protein 21.26%(PD<0.05);each chlorogenic acid group enhanced the content of skirt collagen 13.00%(PB<0.01),25.63%(PC<0.01),17.98%(PD<0.01),27.46%(PE<0.01); 200 and 400mg/kg chlorogenic acid enhanced the content of muscle collagen 56.22% (PC<0.05),54.44%(PD<0.05);200 and 400mg/kg chlorogenic acid reduced the content of muscle cholesterol 13.43%(PC<0.05) and 10.45%(PD<0.05)(5) Chlorogenic acid had good effect on antioxidance of trionyx sinensis. Compared with control group,100 and 200 mg/kg chlorogenic acid enhanced the activity of plasma SOD 27.06%(PB<0.01) and 28.25% (PC<0.01); 200 mg/kg chlorogenic acid enhanced the activity of plasma CAT 118.22%(PC<0.01);200 and 400 mg/kg chlorogenic acid enhanced the activity of plasma GSH-PX 26.18%(PC<0.01) and 44.82%(PD<0.01),200 and 400mg/kg chlorogenic acid reduced the contentof plasma MDA 17.83% (PC<0.05) and 21.02%(PD<0.05)In summary,400mg/kg chlorogenic acid enhanced the growth of trionyx sinensis remarkably; 200 and 400 mg/kg chlorogenic acid enhanced the content of muscle and skirt collagen in trionyx sinensis,and reduced the content of muscle cholesterol in trionyx sinensis obviously; 200 and 400 mg/kg chlorogenic acid increased antioxidant function of trionyx sinensis. As a result,200～400 mg/kg CGA could improve production performance and increase antioxidant function in trionyx sinensis.