The Research Of Calmodulin Regulation On Antioxidative System In The Wheat Seedling Induced By Enhanced Ultraviolet-b Radiation

The wheat plays an important role in the national economy, but as a result of the industrialization process forward, the ozone hole led to UV-B radiation enhancement. Enhanced UV-B radiation cause serious hurt on wheat production, physiological, morphological, molecular Level, biochemical indicator such as antioxidative system, caused great impact on the development of the entire national economy.Calmodulin is a very important signal of the Ca2+ receptor protein, which in the Ca2+ second messenger involved in signal transduction plays a key role in the process, mediated by the entire life cycle of plants. Ca2+/CaM signaling networks through its numerous effects triggered cell growth, development and physiological processes so that plants adapt to environmental changes. However, the relate between calmodulin and enhanced UV-B radiation on the antioxidat ive system of wheat research at home and abroad is still blank, the study of calmodulin enhanced UV-B radiation on the antioxidative system under study makes sense. Therefore, this article on the topics of calmodulin and anti-oxidative system to study in wheat seedlings by enhanced UV-B radiation strive to calmodulin as a starting point to explore the enhanced UV-B radiation on antioxidative system in wheat, the injury mechanism, and enhancing UV-B radiation on wheat, calcium signal transduction pathway was studied, so as to make anti-radiation mechanism of a certain foundation.This paper select to separate and purify Calmodulin mainly uses the secondary salting, Sephadex G-50 gel filtration chromatography methods and PAGE were identified by extracting has reached the electrophoretically pure. At last, anti-oxidative system is determined. The results are as follows:1. In this paper, isoelectric point precipitation, secondary salt precipitation, dialysis and SephadexG-50 gel filtration chromatography methods to isolated from the wheat out of CaM, and determines when the ammonium sulfate salting-saturation. At last, SDS-PAGE were identified by extracting has reached the electrophoretically pure.2. The content of CaM were determined by PDE. The results show that: The CaM content in wheat seedlings enhanced UV-B radiation under normal light levels increased by about 23%.3. Research the changes in calmodulin content of enhanced UV-B radiation and antioxidative systems in vitro. The results show that: enhanced UV-B radiation induced increase in CaM content, its trends and changes in the activity of five antioxidative enzymes (APX, SOD, CAT, GPX, GR) and four anti-oxidative content (AsA/ DHA, GSH/GSSG) changes in are relevant, As well as uses the CaM inhibitor determination antioxidase activeness and the oxidation inhibitor content change, indicating that enhanced UV-B radiation 4. CaM involved in the regulation of antioxidative systems, And Ca2+/CaM through its signal network regulation wheat seedling oxidation resistance system. Thus makes the wheat seedling adaptation outside to enhanced the UV-B radiation change the environment.In short, this article detected CaM from the enhanced UV-B radiation wheat seedlings, and found that their participation in the regulation of anti-oxidative system, indicating that enhanced UV-B radiation on the impact of wheat has Ca2+ signal transduction pathway involvement and regulation.