The Experimental Study Of The Effects Of B-FGF On Multiplication And Differentiation Of Rabbit Osteoblasts And Biological Calcium Phosphate Combined With B-FGF,bone Marrow Repairing The Bone Defects

Tissue engineering is an interdisciplinary field that applies the principles and methods of engineering and engineered and the life science to the development of biologic substitute.We discuss the possibility of the Tissue engineering through the experimental studies of the b-FGF~ osteoblast~ BCP. Part I The experiment discussed effects that different doses of b-FGF worked on the DNA compose and ALP activity of the osteoblasts.The conclusions are as below: b-FGF plays an important role in DNA synthesis and ALP activity of the osteoblasts. Flow cytometer was used to analyse DNA contents of the cells.b-FGF has double effects on the osteoblasts, showed a biphasic dose-response curve, so that 1.0-1 OngIml increased the DNA contents of the cells, but higher or lower doses had no effect. A dose of lOng bFGF per milliliter had the strongest effect on increasing the DNA contents of the cells. We examined the effects of the b-FGF working on the ALP activity of the osteoblasts by the way of enzyme dynamics. We found the b-FGF can inhibit the activity of the ALP. A dose of I Ong b-FGF per milliliter had the most distinct effect. Part II The X--ray diffraction.. multifunction electron miniprobe surface analyzer nuclear magnetic resonance spectrum analyzers infrared spectrometric analyzer were applied to examine the structure of the BCP ,which proved the structure of the BCP was the similar to the structure of the human bone. In vitro, the osteoblasts were cultivated with the BCP, through the observation of the inversed microscope and the surface endoplasmic reticulum, we found the osteoblasts multiplicated well on the surface of the BCP, which suggested that the material has a good biocompatibility. The standarlized 1cm defect in Newzeland rabbit ulna was used to be the model, the BCP combined with bone marrow cells.. b-FGF was implanted to repair the defect. The radiographic and histologic characteristics were investigated. The result was we repaired the defect in Newzeland rabbit ulnas sucsusfully by this way.