| IntroductionFree radicals are short-lived atomic or molecular compounds characterized by unpaired electrons in their outer orbit. These unpaired electrons are the cause for the high chemical reactivity of free radicals. Most radicals of biological importance originate from oxygen or nitrogen. For this reason, the terms reactive oxygen/nitrogen species or intermediates (ROI/RNI) have been employed gradually. Free radicals generated and are scavenged continuously to keepdynamic equilibrium in the body. The continuous generation of free radicals is by enzymatic and non-enzymatic reactions. Free radicals keep an low and stable level in the body physiologically and have diverse important biological functions. Whereas under certain conditions such as certain pathological state ROI/RNI overproduce and scavenge ability of the body is insufficiency, under this circumstance, Free radicals can damage lipid, protein and DNA and result the damage of cells and tissues at last. By this way, free radicals have been manifested to take part in the pathogenesis of a variety of diseases.Acute pancreatitis is one of the commonist acute abdomen diseases that need admission. The causes of AP can be attributed to many mechanical, vascular and metabolic factors and so on. Any ages and strata can suffer from AP. The average mortality of AP can reach 10%. Clinically in the development of AP, AP is often complicated with systemic inflammatory response syndrome (SIRS), and may thus lead to pyemia, multiple organ dysfunction syndrome (MODS) and even death. Whereas the pathogenesis of AP and SIRS still remain unclear up to now. Traditional themry proposed AP is the result of contra-permeation of pancreatic fluid and activation of pancreatic zymogens lead to "pancreatic autodigestion". Nevertheless, Inhibition of digestive enzymes that secreted by pancreatic acinus has unsatisfactory curative effect. On the other hand, with the advances in the research of SIRS, mostly researchers at present have suggested the pathogenesis of SIRS is related with activation of several group cells and uncontrolled release of various inflammatory mediators. In the recent years, many investigations have manifested that a series of pathophysiological alterations caused by the imbalance of free radicals release-clearance mechanism play an important role in the pathogenesis of either AP or SIRS. Aggravatedoxidative stress and superoxidative injuries were found in both patients and experimental animals with AP and/or SIRS, and reduced antioxidative capability also was found, antioxidant Treatment has been proven to be useful in AP and/or SIRS. However, at present mostly researches still stay at animal experiment level and are rather scattered. Furthermore, the purposes of the role of nitric oxide (NO), one of RNI, in the pathogenesis of AP were contrary. Therefore, further clinical researches are necessary,In the present studies, NO and other free radicals and index of oxidative stress in the blood of AP and complicated SIRS patients were determined by spectrophotometrically, and the correlations with the severity of AP were studied. The aim of present studies is to explore the role of blood nitric oxide and other free radicals in the pathogenesis of AP and complicated SIRS, and to reveal the nosogenesis of AP and SIRS from the point of free radical medicine, also to offer the useful supervisory marker of severity of AP for clinic.Materials and methods Materials1. clinical materials: include criteria and exclude criteria were work out according to the clinical diagnosis criteria of acute pancreatitis (the second program) criterionized by pancreaticological group of surgical college of China Medical Association in 1996. 50 cases (26 male and 24 female) of AP were included. There ages were from 6 to 75 (51.6 ?13.3) years. All the patients had never suffered from heart diseases, brain diseases, lung diseases, liver diseases, kidney diseases, diabetes mellitus, autoimmunity diseases, peripheral angiopathy and tumor formerly. 50...
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