| IntroductionThe acute pancreatitis which own the character of cal-amitous pathogenetic condition and high opportunity of morbidity and mortality is a commonly acute abdomen in surgery.Except for impairment of pancreatic enzyme's autodigestion, many inflammatory mediators also participate and develop the natural course of pancreatitis.And at the same time the extent of impairment in extrapancreas organs are become more and more severity.Because of dysimmunity through the course of pancreatitis,the multiple organ dysfunction syndrome is apt to take place.The measurement which modulating pro- and anti-inflammatory cytokines,ameliorating the state of body's immunity and keeping immunologic balance in a lower level is benefit to control inflammation and elevate on prognosis in patients.As previous rat experiment,the inflammatory cytokines such as TNF-α,IL-8,IL-10 and so on in serum of early phase of acute pancreatitis increased predominantly.After treated with COX-2 inhibitor,the pro-inflammatory cytokines such as TNF-a,IL-8,IL-10 significantly decreased.Obvious improvement of index of the severity of the acute pancreatitis,including the blood routine counting,amylase and the secondary injury in lung tissue,also were observed after treatment with COX-2 inhibitor.We don't know whether COX-2 inhibitor can also rectify the abnormal immunity.According to this question,the objective of this experiment is to observe the effect of COX-2 inhibitor on prognosis of Sprague-Dawley(SD) rat and to discuss the probable mechanism of treatment in acute pancreatitis using by COX-2 inhibitor through establishing acute pancreatitis model and selecting COX-2 inhibitor(Celecoxib) as interventional measurements. Materials and methodsFirst,fifty four female 6-7 weeks old SD rats were randomly divided into 3 groups: Group 1:The sham rats as a control;Group 2:Acute pancreatitis model were established by retrograde pancreatic duct injection with 3.3%Sodium taurocholate at the volume of 2ml/kg weight;Group 3:When the models are done,the rats were injected with Celecoxib 1ml from lavage-needlepoint respectively.the rats in three groups were killed at 3,12,24 hours after operation.The ordinary situation and the ascites rate were observed.Blood samples were taken for measurement of blood routine counting,blood amylase,TNF-αIL-8 and IL-10.Pathologic examination of pancreas were also tested.Result1.Twenty four hours after pancreatitis were induced,the leukocyte counting,the blood amylase,the level of TNF-α,IL-8 and IL-10 in group 2 were(16.02±0.98)×109·L-1,(1845.80±297.82)IU·L-1,(190.99±36.99) pg·ml-1,(127.50±11.24) pg·ml-1 and (192.34±7.87)pg·ml-1 respectively.The same index in group 3 were(9.14±0.48)×109·L-1, (708.69±33.29)IU·L-1,(65.89±2.79)pg·ml-1,(61.52±5.03)pg·ml-1 and(92.04±7.80) pg·ml-1 respectively.2.Compared index in group 3 to which in group 2 pre- and post-operation, every index improved significantly(P＜0.05) in 24 hours phase in this experiment.ConclusionCyclooxygenase-2 inhibitor's protective effect may be achieved through rectifying both the level of pro- and anti-inflammatory cytokines and keeping immunologic balance in a lower level.
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