An Experimental And Clinical Study Of The Ralationship Between Cytokine Genotype And Kidney Transplantation

The concrete role of cytokine gene polymorphisms in predicting the effect of kidney transplantation has not yet been completely clarified. It has been reported that tumor necrosis factor-α (TNF-α) is associated with acute rejection following renal transplatation, while transforming growth factor-β1(TGF-β1) is associated with chronic rejection following renal transplatation. Current information concerning interleukin?0 (IL-10) is conflicting. In order to access the relationship between cytokines levels and their genotypes, and to investigate the role of cytokine gene polymorphisms in predicting the effect of kidney transplantation, plasma cytokines levels were observed by the means of enzyme-linked immunosorbent assay with avidin-biotin-HRP (horse radish peroxidase) complex (ELISA-ABC) and polymerase chain reaction with sequence specific premier (PCR-SSP). As a result, the plasma levels of cytokines rose increasingly with the change of cytokines producer genotype from low to high. The plasma level of TNF-α in high producer genotype(56. 63±12. 01pg/ml) was significantly higher than that in low producer genotype(31.58±76pg/ml,P<0.01).The plasma level of TGF-β1 progressively increase with the change of TGF-β1 producer genotype from low, moderate to high(1O15.87±107.61,454.43±76. 59,134. 16±65. 97ng/ml, respectively; P＜0. 01). Similarly, The plasma level of IL-10 progressively increase with the change of IL-1O producer genotype from low,moderate to high(45.13±11.94,24.57±7.46, 13.61±4.82pg/ml, respectively; P＜0.01).A greater proportion of the recipients with TNP-αhigh producer genotype had acute rejection(61.76%)compared with recipients with the TNF-α low producer genotype ( 24. 55%, P＜O. 001) .Similarly,a greater proportion of recipients with IL-10 high producer genotype had acute rejection compared with recipients with the TNF-α low producer genotype (P<0. 01) . A greater proportion of the recipients with the TNF-αhigh/IL-10 high producer genotype (3/3, 100%) had acute rejection as compared with all other genotypes (24/72, 33. 33%; 0. O1O. 05) . Similarly, a greater proportion of recipients with IL-10 high producer genotype/CsA low dosage (11/15, 73. 33%) had acute rejection compared with recipients with the other IL-10 genotype/CsA dosage (28/111, 25.23%; P<0.01) .Otherwise, no associations were found...