The Study Of Ribavirin Proliposomes

A modified rotary evaporation unit was employed for the manufacture of ribavirin proliposomes from natural soyabean lethicin. The uniform design was used to obtain the optimized prescription. The Entrapment Efficiency (EE) of Ribavirin proliposomes after hydration was determined and some factors which may affect the Entrapment Efficiency such as the size of the proliposomes, the intensity and the time of hydration were investigatedThe physicochemical properties of ribavirin proliposomes were studied. The content of ethanol residual was only 0.0 12% contrasted with 5% of that prepared by ethanol injection method. The morphological characteristic of proliposomes samples before and after hydration was revealed under scanning and transmission electron microscope. Upon hydration, ribavirin proliposomes formed multilamellar liposomes with a number equivalent diameter of 300.8nm. in vitro release of ribavirin proliposomes in different dissolution medium was studied by a dialysis method. Differential Scanning Calorimetry of proliposomes revealed the phospholipid endotherm to give a phase transition temperature of ?1.19 ~D .The surface electric potential, pH, viscosity, and the??M.S. Thesis of Sheriyang Pharmaceutical UniversityAbstractsurface tension of hydrated liposome was ?30.62mV,5.20 .1.2 mPa s and 44.15 X 1 &NIm respectively. The results in stability test indicated only minor changes in Entrapment Efficiency and content as a function of time.The experiment of irritation and acute toxicity of proliposomes was carried out and proved that liposonies could be safely administrated. The pharmacokinetics and tissue distribution of ribavirin proliposomes were studied after po and intranasal administration ribavirin proliposomes to rats. The pharrnacokinetic parameters were computed according to nonapartment model. The relative bioavailability and drug targeting index (DII) of ribavirin proliposomes compared with ribavirin solution was computed and evaluated in two route of nonparenteral administration. It is shown that although proliposome did not promote the drug to penetrate the blood-brain barrier (BBB), it is feasible and effective on oral and intranasal absorption.