Frequency Of BsiY I (exon 1) And Taq I (intron 4) RFLP Of Endothelin-1 Gene And Its Relationship To Pregnancy-induced Hypertension And Ischemic Stroke In The Chinese Han Population

Background: Endotlielin-l gene is the most potent vasoconstrictor peptide originally characterized from a culture supernatant of porcine aortic endothelial cells. It has been founded that endothelin-l gene plays a role in the pathogenesis of pregnancy-induced hypertension and ischemic stroke. Objective: We have studied that if there are I3siY I polymorphism and Taq I polymorphism of endothclin-l gene in Chinese I-lan population and the difference and correlation between the normal controls and the women with pregnancy-induced hypertension/ischemic stroke and whether the frequencies of the RFLPs are contributed to these two diseases. Methodology: We extracted the geiionie DNA from leukocyte as PCR templet,use PCP-RFLP to document the polymorphism of l3siY I in exon I of endothelin-l gene gene and polymorphism of Taq I in intron 4 of endothelin-l gene. Results: (1) Detecting the site of BsiY I in exon I of endothelin-1 gene g~. There was no subject with BsiY I polymorphism in 70 random controls of Chinese Han population. (2) Detecting the site of Taq I in intron 4 of endofhelin-l gene gs~€ 3 a. The study of the relation between the polymorphism arid the women with pregnancy-induced hypertension The research group has been divided in three groups. The frequency of C allele is 75.3% and D allele is 24.7% in 166 alleles in random women controls. The frequency of C allele is 71.8% and D allele is 28.2% in 170 alleles in normal pregnancy women. [lie frequency of C allele is 56.4% and D allele is 43.6% in 110 alleles in the women with pregnancy-induced hypertension. b. The study of the relation between the polymorphism and the people with lschernic stroke The research group has been divided in three groups. The frequency of C allele is 68% and D allele is 32% in 178 alleles in random controls. The frequency of C allele is 74.3% and D allele is 25.7% in 140 alleles in healthy controls. The frequency of C allele is 72.4% arid D allele is 27.6% in 174 alleles in the people with lschemic stroke. Conclusion: (1) There is no polymorphism of I3siY I polymorphism in exon I of endothelin-l gene in Chinese Han population. (2) There is Taq I polymorphism in intron 4 of endothelin-1 gene in Chinese 1-lan population. (3) Statistically significant difference has been found between the normal controls and the women with pregnancy-induced hypertension. it shows that there is associated between the Taq I polymorphism of endolhelin-1 gene and P11-f. (4) No Statistically significant difference has been found between the normal controls and the people with ischemic stroke. It shows that there is rio associated between the B~sPi~-4 polymorphism of endothelin-l gene and ischemic stroke. This study has been found in China up to date.