Treatment Of Murine Hepatocarcinoma By FLT3 Ligand Recombinant Adenovirus Combined With Adriamycin

At present cancer is one of the most dangerous disease and liver cancer is called 搕he king of the cancer? whose death rate is very high. Now the treatment of cancer is based on operation plus chemotherapy or radiation therapy. Since these methods are harmful to immune system and hematopoietic system, their curative effect cannot be satisfied. So recent years gene therapy becomes a hot spot of the field, but its clinical effect is not pleased . At the moment , gene therapy still cannot replace the conventional methods. However, research indicates that gene therapy can strengthen immunity, improve chemosensitivity, decrease the relapse and metastasis, so how to combine the gene therapy with conventional methods effectively becomes the main point of cancer research.The flt3-ligand (FL)is a recently identified cytokine ,whice can stimulate the proliferation of hematopoietic progenitors and enhance immune system. We hope that combination of FL and chemotherapy can relieve chemical drugs side effects on immune system and hematopoietic system , in mean time enhance its anti-tumor effect ,so we designed the followed experiments:1.Tested the chemo-sensitivity to anti-tumor agents in mice hepatocelluar carcinoma using a hepal-6 liver cancer cell line;2.Cloned the murine FL and constructed the recombinant adenoviral vector, paving the way for further cancer immunotherapy;3.Treated hepatocarcinoma in mice using murine FL recombinant adenovirus combined with chemotherapy.The results show:1.Adriamycine (ADM) and 5-fluorouracili (5-FU) are more effective to inhibit the growth of hepal-6 liver cancer cells;2.FL combined with ADM could significantly inhibit the growth of the liver cancer , make the rats survive long;3.FL could relieve ADM toxic effects on hematopoietic system ,make it recovery earlier.3-Domestic research on FL is focus on expansion of human cord blood cells in vitro ,not on its anti-tumor effects .Other countries have no reports on FL recombinant adenovirus activity against hepatoma , esp. on anti-tumor activity of FL combined with chemotherapy .We hope that these experiments can offer a new way for clinical cancer treatment and offer a theory for FL used in clinical treatment...