Studies On The Expression Of Mac-1 And Regulated By Calcium Antagonist During PMN Primed And Activated After Trauma In Rats

The P2 integrin CD li/CD 18 which are constitutively expressed on the surface of ieukocytes are heterodimers consisting of a common P subunit (CD 18) and an i subunit (CD 11 a,CD 11 b,CD 11 c) .They mediate cell-cell and cell-matrix contacts by binding their specific ligands allowing firm adhesion, spreading, and subsequent emigration of leukocytes. They play a fundamental role in cell growth,differentiation, inflammation, injury healing. Polymorphonuclear nuetrophil (PMN)adhesion is a major event of the early inflammatory response that is characterized by the recruitment of nuetrophils into areas of inflammation,which begins with the binding of these cells to endothelium cell(EC) followed by their transmigration into tissues.Distinct phases have been characterized during nuetrophils binding, including rolling, activation, and firm adhesion. The molecular basis for these phases is the up-regulation of various cell adhesion molecular that belong to three major families: the selectins, the P2 integrins, and the immunoglobulin superfamily, the selectins are important for rolling, whereas firm adhesion of is primarily P2 integrins-dependent. Mac-i (CD 11 b/CD 18) appears to be the major P2 integrins responsible for the firm adhesion of nuetrophils to endothelium cell and myriad extracellular matrix molecules. The possible three dimensional structure of Mac-i showing the major defined regions of its a -subunit(CD1 lb). Nuetrophils exist in one of three states: quiescent, primed or activated. Activated-nuetrophils are a double-edged sword, it play important roles in host defense against all classes of infectious agents but, paradoxically, they are also involved in the pathology of various inflammatory conditions. In the primed state, there is no increase in oxidase activity, yet subsequent stimulation provokes a response that is larger than in nonprimed,activated cells.Thus,individual primedcells may be thought of as being "ready to go" but awaiting further stimulus beforethe oxides response is elicited. The primed nuetrophils are thus held at "amber",awaiting "green"before activity is triggered.Recent stUdies have demonstrated that cell,tissue and organ of organism haveamplificational effect on secondary i s chemi a/reperfus io n, metabo l ism o r stre s s o finflammation. Thus, the concept termed as cell priming aPpears,it is refered aspriming that a cell has prepared to first hit. As a first hit, trauma make PMNprimedthat very commonly happens. A large amotmt of PMN were simultaneity activatedand excessiveIy activated, firm adhension to vascular endotheliurn. Thesequestration aPparently elevated in tissue and organ in earlyWhich are mainlycause of induing SIRS,MODS and evenly MOF. As a second messenger, thecytosolic free Ca2+ involved in the pathophysiological changes by PMN mediated aftertrMa.It is lmown tha calcium anagoniSt have protective effects on biomembrane bythe inhibition of Ca2+ iallux reactive oxygen PrOduction and oxylase release.Toelucidate the function of Leu-CAMs, nuetrophils adhesion activity and the effects ofcalciurn anagonist on them during PMN primed and activated after traUIna. We Studiedthe changes of PMN CDllb, PMN-EC adhesion activity and theraPeutic effects ofcalcium antagonist during LPS challenge the PMN harrested at various times frOm ratswith multiple trauma, Which will be valuable in providing theoretical instrUction forclinical trCaunent,We exPect that will find a new road for prevelltion and trCatrnent ofsevere injUry by exploring the mechbosm and biomarker of PMN priming. In our study,adopting murine modles of multiple broken-bone and soft tissue injUry The methodsused in our study are as follows: l. In vitro release O2- of PMN was messured by...