| Experimental study of pathologic and limb function after ischemia-reperfusion in rabbitspinal cord ABSTRACTObjective: To investigate the pathologic changes and resumption of posterior limb function at different time point after ischemia-reperfusion in rabbit spinal cord by selectively blocking lumber, explore the potential association between them, and provide evidence for clinical treatment by identifying the precise time course of ischemia which resulting in mock-up complete paralysis and incomplete paralysis in animal model. Method: 36 New Zealand white rabbits were divided into 5 groups according to time course of ischemia(30minutes, 45minutes, GOminutes, 75minutes, QOminutes, respectively). Pathologic assessment of spinal cord was measured at 24hours, 48 hours, 72hours, 7days after ischemia-reperfusion injury. Functional assessment with Tarlov' s score and Jacobs Rank were performed.Results: According to the pathologic assessment, spinal grey substance was markedly damaged after spinal cord ischemia-reperfusion. The degree of damage was aggravating with the prolongation of ischemic course. The functional rank andscore was coincided with the severity of spinal cord damage.Group with ischemia 30 minutes were suffered from mild paralysis, Group with ischemia 45 and 60 minutes were suffered from moderate paralysis, Group with ischemia 75 minutes were suffered from severe paralysis, Group with ischemia 90 minutes were suffered from complete paralysis. The function of posterior limb in group with 60 minutes was worse than that of 45 minutes ischemia.Conclusion: 1. It is a good choice of making animal model of spinal cord ischemia-reperfusion to block the lumbar. 2. The pathologic changes and the damage of posterior limb function are in direct proportion to the duration of spinal cord ischemia. 3.It help to consider the groups whose ischemia lasting 45 and 60 minutes as the animal model of incomplete paralysis.
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