| ObjectiveEstablish the stable lumbar spinal cord ischemia model with all lumbar artery ligation in Sprague-Dawley(SD) rats, then observe(in 48h) the early changes of the hindlimb motor function and lumbar spinal cord ischemia. For further study of spinal cord ischemic injury mechanism and drug therapy. Method66 specific pathogen free level(SPF) SD rats(male) were randomly assigned to 2 groups, control group(12 rats): just separate out all lumbar arteries; ischemia group(54 rats): lumbar arteries were separated out and ligated.Choose 6 rats randomly from control group, and the Basso Mouse Scale(BMS) scored in 1h, 2h, 4h, 6h, 8h, 12 h, 24 h, 48 h after the operation to evaluate hind limbs locomotion function. Ischemia group, the BMS scored in 1h, 2h, 4h, 6h, 8h, 12 h, 24 h, 48 h after the operation, choose 6 rats randomly each time to evaluate hind limbs locomotion function.Choose the last 6 rats of control group at 0h after operation, the last 6 rats of ischemia group at 0h after operation and at 1h, 2h, 4h, 6h, 8h, 12 h, 24 h after BMS scored, abdominal excessive anesthesia executed in rats, then using the Triphenyltetrazolium chloride(TTC) staining of the lumbar spinal cord to evaluate staining area and position.Correlation analysis of TTC staining with BMS using Pearson correlation coefficient. Result Control group BMS score at 1h post-op. was(8.83±0.52), at 2h was(8.83±0.41), at 4h, 6h, 8h, 12 h, 24 h, 48 h was(9.00±0.00), differences had no statistical significance(P>0.05). Ischemia group BMS score at 1h, 2h, 4h, 6h, 8h, 12 h, 24 h, 48 h was(0.25±0.41),(1.05±0.94),(2.08±1.16),(2.97±1.16),(4.29±1.52),(6.22±1.11),(8.08±0.79),(8.83±0.41), only 8h and 12 h, 24 h and 48 h comparison differences had no statistical significance(P >0.05), all the other comparisons have a statistically significant difference(P<0.05). BMS score comparison between control group and ischemia group, differences between the two groups have statistical significance(P<0.05). Only at post-op. 48 h, differences had no statistical significance(P >0.05).TTC staining area: control group at 0h was(0.95±0.02)%, ischemia group B at 0h, 1h, 2h, 4h, 6h, 8h, 12 h, 24 h, 48 h was(0.04±0.01)%,(0.37±0.06)%,(0.61±0.07)%,(0.64±0.07)%,(0.70 ± 0.07)%,(0.72±0.05)%,(0.76±0.02)%,(0.89±0.02)%,(0.94±0.02)%.Compared control group at 0h with ischemia group, there has statistics difference(P<0.05), except ischemia group at 48h(P>0.05). Compared ischemia group B at different time, 0h with 1h, 1h with 2h, 4h with 6h, 12 h with 24 h, 24 h with 48 h, the differences had statistical significance(P<0.05). Compared the upper section, middle section, lower section of staining area in ischemia group, the upper section>middle section>lower section, the upper and lower, middle and lower differences had statistical significance(P<0.05).The correlation between BMS and TTC staining in ischemia group is respectively analyzed by Pearson correlation coefficient, the Pearson correlation coefficient>0.8. ConclusionThis study on rats lumbar artery ligation, then observe the function of hind limbs using BMS system and lumbar myeloid ischemia by evaluating TTC staining within 48 h, the results show that: After the SD rats lumbar arteries were ligated, which can cause lumbar spinal cord ischemia and hindlimb function lost; the spinal cord ischemic and function recovery in 48 h, and recover from the upper lumbar spinal cord; the Pearson correlation coefficient indicate that lower limb motor function recovery and lumbar myeloid blood supply recovery has obvious positive correlation, hind limbs function recovery later than lumbar spinal cord blood supply recovery, and the function recovery with the blood supply recovery; the model has good stability and recover soon, is suitable for lumbar spinal ischemia and recovery research. |
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