Study On The Correlation Of Costimulatroy Molecule B7 And Transforming Growth Factor-betal In Pathogenesis Of Endometriosis

Objective To study the relationship between expression of B7-land B7-2 in endometrium and endometriotic tissueEndometriosis is a benign gynecologic disorder, though aggressive, disease of the female reproductive tract that consists of endometrial stromal and epithelial cells growing at an extrauterine site. A more thorough understanding of the mechanisms associated with the cause and pathophysiology of endometriosis may help in the development oft new diagnostic and therapeutic methods for the management of?endometriosis. Research has begun to enhance our understanding of endometriosis by demonstrating the differences and similarites between eutopic and ectopic endometrium, and by characterizing the peritoneal environment. It is a disorder that markedly affects well-being and physical and emotional health in women. Research on the pathogenesis of endometriosis currently interfaces with four areas of basic research, including the fields of genetics, environmental science, cancer biology and immunology. Here we focus on research in the last discipline.A general property of lymphocytes, including both T and B cells, is the need for two distinct extracellular signals in order to induce proliferation and differentiation into effector cells. The first signal is provided by antigen binding to the antigen receptor. In the case of T cells, peptide-MHC complex binding to the TCR (and CD4 and CD8 coreceptors) provides signal 1. The second signal for T cellIIactivation is provided by costimulatory molecules, which are surface molecules on the T lymphocyte. One of the most important and best characterized costimulatory phthways in T cell activation involves the T cell surface molecule CD28 , which binds the costimulatory molecules B7-1(CD8O) and B7-2 (CD86) expressed on APCs. CD28 delivers signals that enhance T cell responses to antigen. In addition, another T cell surface molecule, CTLA4, also binds B7-land B7-2 but in contrast to CD28, it transmits signals that inhibit T cell activation.Method Use the Anti-CD80(B>1),Anti-cD86(B72) to determine the expression of B7-1 , B7-2 in eutopic and ectopic endometrium.Result Increased concentrations of B7-2 in endometriotic tissue. Expression in all endometriotic samples. Thus suggesting that it could have an important role in the maintenance and propagation of the disease. This could be a mechanism for endometrial cells to escape immune surveillance, implant and growing. Peritoneal fluid was obtained from patients with endornetriosis during laparoscopy or operation. B7-1(CD8O) and B7-2(CD86) could be detected in all women presenting with various stages of active endometriosis(according to the rAFS score). Patients with stage III and lVendometriosis had much higher concentrations of B7-1 and B7-2 compared with mild endometriosis(rAFS stages I and II). Interestingly the concentration of B7? in stage 1 and 2 was higher than in stage 3 and 4 with this disorder.Conclusion The results suggested that the expression of B7-1,B7-2 in endometrium were different in endometriosis and non?endometriosis.