| Objective:Investigate the effect of hyaluronan(HA) and hyaluronanidase(HAse) on wound healing ang the formation of hypertrophic scar in rabbit ear after injuryMethods:Wound healing models were established on 18 adult rabbit ears, which were randomly divided into the HA treated-group(group a), the HAse treated-group(group b), the PBS treated~group(group c). The process of wound healing was observed morphologically and histologically. The expression of fibronectin and a -smooth muscle actin were detected by imunohistochemistry. The variation of the contents of hydroxyproline(HPr) at the stage of wound healing and post healing was measured The relative height of scars were measured at the peaks of scar formation.Results: 0The mean values of wound healing time of21group a, b and c was 11.7 + 0.6, 9.8 ?.9, 10.8 + 1.0 days respectively. (2)Compare with PBS controls and HAse treated group, the collagon fibril was slender and arrayed in good order in HA treated wound at early stage of wound healing. But there were no difference among them at the stage of scar formation. (3) The contents of HPr were statistically lower in HA treated group than in controls at the stage of wound healing, but no difference at the stage of scar formation The HPr contents in HAse treated group were higher than the others all the way. (4) The express of fibronectin and a -smooth muscle actin in group b was greater than group a ang c. And, wounds of group c contract fast and prominent . Wounds of group a act diverse to group b. (5)The mean values of relative height of group a, b and c were 1.55 ?0.35, 1.89?.39, 1.59?.29 respectively;Conclusion:(1)Exogenous HA on the wound surface delay wound healing and failed to affect the scar tissue proliferation,because of unable to panetrate the epiderms (2) HAse accelerate wound healing and the scar tissu proliferating... |
PDF Full Text Request