The Changes Of Metabotropic Glutamate Receptor Subtype MGluR1a After Diffuse Brain Injury And Secondary Brain Insults And The Effect Of Its Antagonist

Metabotropic glutamate receptors (mGluR) ,which are coupled through G-proteins to second-messenger systems, are one kind of glutamate receptors. Molecular cloning techniques have elucidated eight mGluR subtypes to date. Recent studies about excitotoxicty of glutamate were mainly focused on ionotropic glutamate receptors( iGluR). In contrast, little is known about the role of mGluR after diffuse brain injury(DBI), especially when this injury is coupled with secondary brain insults(SBI). In the present study the changes of metabotropic glutamate receptor subtype mGluRta in Marmarou?s rodent model of impact acceleration diffuse brain injury with SBI and the effect of its antagonist a-methyl-4-earboxyphenylglycine (MCPG) were observed. The study is divided into two parts: 5 Part I The changes of metabotropic glutamate receptors subtype mGluRia and the effect of its antagonist MCPG after diffuse brain injury First, we establish Marmarou抯 rodent model of impact acceleration diffuse brain injury,165 male SD rats were randomized into A and B two groups. Each group was sub-divided different subgroups. After Marmarou抯 rodent model of impact acceleration diffuse brain injury, rats were decapitated as planned and the cerebral cortex mGluR1 a was examined by immunohistochemistry, RT-PCR and pathological method . RESULTS: 1. At lb after injury the expression of mGluRl a increased and the most robust increase occurred at 24 h after injury in the injured cerebral cortex. (P