| Objective: To try to find out laws of cytokines (including IL-6, IL-8, IL-10 and TNF- a) occuring and developing both during inflammatory response to cardiopulmonary bypass and under intervention treatments, while to observe the every patient抯 clinical manifestations of inflammatory response to cardiopulmonary bypass at the same time. Methods: 30 patients having diagnosed as Cl-ID VSD ( congenital heart disease, membranous ventricular septal defect ) were randomized into A (Aprotinin, 10 cases), M ( Methylprednisolone, 10 cases) and C (Control, 10 cases) group. They all were balanced into 3 groups according to 憄rinciple of the least fractions adding up?by age, sex, body weight, using aortic cross-clamp or not and VSD needing patching or not during operations. In group A, Aprotinin (2,500,000KW ) were added to extracorporeal circulation machine and in group M, Methylprednisolone ( 200mg ) were also given into the heart-lung machine, while in group C, only the standard anaesthetic techniques and operative procedures were to be used. In each group, blood samples were taken from the radial artery at the following times: after induction of anaesthesia, 10 minutes after the start of CPB, and the 1st, 3rd and 7th day of post-operation ( 7AM ). Blood ( 2ml every time ) was drawn into EDTA ( ethylene-diamine-tetraacetic-acid ) tubes. 8 After centrifugation for 10 minutes ( 2000g at 400), plasma samples were frozen immediately in aliquots at ~300C and were thawed only before testing. The first part, experiment investigation, cytokines levels determined by enzyme-linked immunosorbent assay ( commercial test kits of YES BLOTECH LABORATORIES LTD, Canada ). All values were measured twice, extinction rate was determined at a wave length of 405 nm. The second part, clinical study, all clinical markers, involving functions of breath and circulatory system and others, were be recorded at the same times. All data were given as mean + standard. For a comparison between the groups, analysis of variance (Repeated Design ANOVA, SAS 6.12 ) was performed. A P value of less than 0.05 was considered to indicate statistical significance. Resu1ts:.1b.e....fir~ part experiment investigation IL~ There are statistical significances between group A and C (A: 40.78+16.l9pg/ml, C: 135.45+86.97pg/ml; P0.0004) and group M and C (M: 56.67+24.24pg/ml, C: 135.45+86.97pgIml; P0.0025) at 30 mm after the end of CPB. JL~8 There are statistical significances between group A and C (A: 82.34?3.42pg/ml, C: 134.61+41.93pg/ml; P0.00l7) and there are no statistical significances between group M and C (M: 106.12+32.57pg/ml, C: 134.61+41.93pglml; P0.0680) at 30 mm after the end of CPB. There are statistical significances between group A and C (A: 21.81+6.37pg/ml, C: 30.98?1.47pg/ml; P==0.0310) and group M and C (M: 21.79+8.46pg/ml, C: 30.98+11.47pg/ml; P0.0305) at the 1st day of post-operation. There are statistical significances between group A and C (A: 4l.86+14.35pg/ml, C: 75.39+31 .43pg/ml; P0.0080) and there are no statistical significances between group M and C (M: 63.83+29.38pg/ml, C: 75.39?1.43pg/ml; P0.3322) at the 3rd day of post-operation. J.L4Q There are statistical significances between group A and C (A: 106.62+30.56pg/ml, C: 57.83+32.7Opg/ml; P=0.0026) and group M and C (M: 93.30?5.38pg/ml, C: 57.83+32.7OpgIml; P0.0232) at 10 minutes after the start of CPB . There are statistical significances between group A a...
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