The Killing Effect And Morphological Study Of Ultrasound And Hematoporphyrin Derivatives On S180

There are three kinds of diseases studied hotly nowadays, that is cancer, cardiovascular disease, immune system disease. However, the study of cancer is noticed more and more with its mortality ranked the first. At present, many treatments have been invented in order to be antitumor. These methods include an application of ultrasound including high intensity focused ultrasound (HIFU) surgery, ultrasound thermal treatment and sonodynamic therapy (SDT). There are some deficiencies and difficulties to apply the first two to clinic, but the third treatment has its advantages. It has coordination effect combined used with the photosensitive drugs (such as hematoporphyrin) and can penetrating tissue deeply and being easier to focus, not need expensive equipment and avoiding thermal side-effect, so the application of sonodynamic treatment is a promising way on treatment of tumors in deep tissue. In order to apply this method to clinic, and treat tumors in human's body with effectively and conveniently activate photosensitive drugs, based on the internal and external studies, this paper is focused on the killing effect and developing process of SI80 ascitic tumor and solid tumor in order to accumulate lots of datas on the study of SDT mechanism.This paper has designed to solve two significant problems. One is to define the optimum hematoporphyrin concentration, the optimum timing of the irradiation and the optimum ultrasonic intensity under firmed ultrasonic frequency, the other is to study whether or not SDT can cause cancer cells apoptosis phenomena in vivo .Main conclusion as follow:1.The effective lower intensity and time of treatment on SI80 are screened. HpD has little killing effect. US has certain killing effect, US with HpD can inhibit the tumor growth effectively.2.The tumor structure by lighter microscopy and transelectric microscopy is observed. The destroy on tumor can be seen first on membrane system and genetic information system.3.The activity of cytochromeC is detected. The paper makes conclusion from the changes of the activity of cytochromeC that the destroy on enzyme system is one of the most important factors to the death of tumor cells.4.The structure of SI80 ascitic tumor after treatment is studied on the microvillus and other superficial changes. Most of cells have the characters of apoptosis.