The Effect Of Handportable Mobiletelephone Microwave Radiation On Rat Central Nervous System

The increasing use of handportable mobiletelephone has raised concerns about possible health hazard effects.lt was well known to us, the handportable mobiletelephone microwave radiation caused broad-spectrum biological domino effect to organism ,especially its non-thermal effect.But the mechanisms of non-thermal effect has not been elucidated. From molecule level to realize the radiation traumatic mechanism and biological effect of handportable mobiletelephone microwave, it would not only maintenance^ the health of greatness handportable mobiletelephon consumer, but also provided experimental data about useing handportable mobiletelephone rightly. Measurement of lactate dehydrogenase (LDH) activity released to the extracellular bathing media has been found to be a simple yet quantitative method for assessing neuronal cell injury in cortical cell culture. Extracellular LDH was both chemically and biologically stable; the magnitude of LDH efflux in the cultures correlates in a linear fashion with the number of neuronsdamaged by microwave exposure. Apoptosis was necessary in development of vital process and is very important in maintaining normal physiological function of organism. Bcl-2 (B cell lymphoma/leukemia 2) and Bax (Bcl-2 associated x) were the regulator genes in apoptosis and played a important role in the occurrence of apoptosis. Bcl-2 encoded a 26KD protein, which localized to outer mitochondrial membranes. Overexpression of Bcl-2 can blocked occurrence of apoptosis. Bax encoded a 21 KD protein. Bax protein could form homodimer. On the contrary, overexpression of Bax could promote occurrence of apoptosis. The task adopted cell culture combined with animal experiment. The neuron were radiated with microwave ,which was frequency of 900MHz and power density of 0.025 mW/cm2, 0.050mW/cn^ 0.100mW/cm2 for 4, 8> 12 ^ 16^ 2Q* 24hours,and the LDH in the media and the activities of neurons were assayed. The healthy male SD rats were radiated with microwave ,which was frequency of 900MHz and power density of 0.050mW/cm2 for 2 h each morning and afternoonjasting 21 days. Then immunohistochemisty were used to detect Bcl-2 ^ Bax expression in all the brain tissue.Materials and methods(? cell culturel.Cell culture Primary dissociated cell cultures were prepared from cerebral cortex of fetal rats.The tissue was dissected, incubated in 0.25% trypsin for 30min.The isolated brain cells were plater at a density of 1 X 106/ml in L-polylysine-coated 12 -well plates.Cells were maintained in growth mediumconsisting of 85% DMEM,15% fetal bovine serum at 37癈 in 5%CO2/humidified atmosphere.The medium was changed twice weekly^2.Microwave paramete: frequency of 900MHz and power density of 0.025 mW/cm\ 0.050mW/cm\ 0.100mW/cm2.3.Radiation method Experiments were performed in 10-12day- old cultures. In cell culture case, the neuron were radiated with microwave for 4 ^ 8> 12> 16^ 20? 24hours.4.Detection: LDH level were measured by colorimetry test and the activities of neurons were measured by trjpan blue straining test(TBST).5.Observation: Then we taken count of 500 cells in convert discrepancy microscope. The pigmentation cell was death.we accounted the percentage of pigmentation cell is the cell mortality (JH) Animal experiment1. Animals: 80 healthy male SD rats (Body weight: about 200g) were divided into 4 groups in random: the group without treatment, the group of cranial defect, the group of normal radiation and the group of cranial defect radiation.2.Craniectomies ; 2 groups were opened a bone window (diameter.4.5mm) in left parietal bone.To avoid the operation's influence,the next experiment would taken after 72 hours.3, Microwave paramete. frequency of 900MHz and power density of 0.050mW/cm2.4x Radiation method: the group of normal radiation and cranial defect radiation were radiated for 2 hour each morning and afternoon,lasting 21 days.5x Material: 5 rats were killed respectively in four groups at CK 24 > 72^ 168hours (7 days) after radiation. Th...