Microcirculation Blood Flow Quantitative Measurement System

The blood flow rate in micrangium an important characteristic index in microcirculation research, and it has important meaning in physiology, pathology and pharmacology.There are a lot of methods to quantitatively measure this data, but all need specific instruments. Now we use common fluorescent microscope to do it. In our research, marked autologous fluorescent blood red cell is immitted into SD-rat body and the whole process is shown and recorded by microscope image system. After these processes, we can replay the recorded tape and sample images with video image card. Then, We use sequence image processing to analysis the image of dark ground microscope. At last, we get the data of the flow rate of microcirculation.We have applied this system in the research of microcirculatory disturbance, and have got some very useful information of the change in microvascular. These information help us to grasp the physiology metamorphose of SD-rat in this status, at the same time these information can assist us to screen some medicine. There will be good result when we combinate thrombus experiment ,Morris water maze experiment with this system.