A Study On Monoclonal Antibodies Against Epithelial Cell Of Endolymphatic Sac In Guinea Pigs

First Affiliated Hospital, Fujian Medical University, Fuzhou 350005 Abstract Using the microdissected endolymphatic sacs of guinea pig (n=35) as an initial antigen preparation. A serial of monoclonal antibodies were estabished, which were used to label the epithelial cells of endolymphatic sac. The antibodies showed strong immunoreactivity with kidney, but no obvious cross reaction with other 15 organs, such as cerebrum, urinary bladder, gall bladder, lung, liver etc. These antibodies included isotopes IgG[, IgG2b and IgM. The results of Western blotting and SDS ?PAGE indicated that the epitopes of monoclonal antibodies in epithelial cells were proteins or glycoprotein with a molecular weight of approximately 74,000. These locations of the epitopes in epithelial cells suggest that they may play some important roles in construction and function of the endolymphatic sac.