| Objective:To explore the distribution of neural stem cell in brain of bature rat the expresstion and proliferation of neural stem cell induced following brain injury of mature rat. Methods: First, observing the disturbution of neural stem cell by Nestion immunostanining of brain of the mature rat ; Second, the cortex and and caudate nucleus of mature SD rats were cut transbersely in stereotaxic apparatus and animals were killed in 1 week, 2 weeks. 3 weeks and 4 weeks following injury. The reaction pattern of neural stem cell after brain injury, The reaction pattern of neural atem cell after brain injury was observed by Nestin immunostaining and Nissl staining. Results: there are a lot of neural stem cells appera in the suben tricular zone (SVZ) at the damaged side, the oppose side and the third brain ventricle, especially on about 1 week. The expression and proliferation of neural stem cells can also be found around the wound, especially in about 2 weeks. The expression and proliferation of neuralstem cells shows a gradient pattern around the wound. Conclusion: The neural stem cell usually stay in the SVZ in the brain of mature rat. The damage of brain can induce the expression and proliferation of neural stem cells around SVZ and wound, and neural stem cells play a chief role in repairing.
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