| Objective : To determine the expression of MatrixMetalloproteinases(MMPs) , Tissue Metalloproteinase Inhibitors(TIMPs) , adhesive molecule E-cadherin and Factor Vffl in lung cancer Specimens, try to find out the possible roles of MMPs and TIMPs in the infiltration and metastasis of lung cancers.Methods:The expression of MMP-1, MMP-2, MMP-9, MMP-13, TTMP-1, TIMP-2, Factor Vffl, E-cadherin were detected in 104 lung cancer tissues by immunohistochemical method, and 5 normal lung tissues as control.Results:1. The expression levels of MMP-1 (81.7%), MMP-2(76.9%), MMP-9(75.0 %), MMP-13(53.8), TIMP-1(80.8%), TIMP-2(67.3%)in the lung cancer tissues were significantly higher than those of the control groups(P<0.005). But the expression of E-cadherin was significant lower in lung cancer tissues(63.5%) than in normal tissues( 100.0%).2. Almost all big cell lung cancer specimens were positive with MMP-1,MMP-2,MMP-9 and TIMP-2, only two tissues were nagative with MMP-13 and TTMP-1.3. In small cell lung cancer tissues, the positive rate of MMPs and TIMPs range from 23.3%?3.3%, and for E-cadherin, it was very low.4. The positive rates of TIMP-2 hi adenocarcinoma was remarkably higher than those hi squamous cell carcuioma(P<0.05), of E-cadherin it was lower in adenocarcinoma than in squamous cell carcinoma.5. Of MMP-2, it was higher in low differentiated lung cancer tissues than inhigher differentiated tissues.The higher expression of MMP-9 was correlated with node metastasis of lung cancer.6. The positive rate of TIMP-1 was significantly higher in tumors with lymph node metastasis than those without lymphatic metastasis (P<0.05), and also higher in stage HI + IV lung cancer tissues man those in stage I + II lung cancer tissues.7. The expression of MMP-2 was significant correlated with it's inhibitors TIMP-1 and TIMP-2, and the expression of MMP-9 and TIMP-2 were also correlated.8. The expression of MMP-1 was significant correlated with E-cadherin and MMP-2 was correlated with microvessel density in lung cancer tissues.9. The expression of TIMP-1 and TIMP-2 was found correlated in lung cancer tissues. MMP-2 was found positive correlated with the expression of MMP-1 of lung cancer (P<0.05).Conclusion:1. The expression of MMPs and TIMPs were up regulated in lung cancer tissues. In different types of lung cancer, the expression of MMPs and TIMPs were different, with big cell lung cancer has the highest positive rate and small cell lung cancer has the lowest positive rate. Adenocarcinoma have higher positive rate for almost all the MMPs than squamous cell carcinoma, which perhaps can explain why adenocarcinoma has the more liability to metastasis to the remote site than squamous cell carcinoma. The different expression of MMPs in different types of lung cancer need further investigation and also its mechanism and relavent functions.2. The positive rates of TTMPs were also higher than that of the normal lung tissues, and the expression of TIMPs was correlated with MMPs, form which we conclude that perhaps TIMPs were up regulated by some mechanisms in response to the high expression of MMPs, so as to resist thefunctions of MMPs. Also, TIMP-1 were correlated with differentiae degree and node metastasis of lung cancer. So perhaps TTMPs can serve as indicators of tumor prognosis and also as a marker of early diagnosis.3. By cooperation, different MMPs played different roles in the infiltration and metastasis of lung cancers, include degradation of extracellular matrix, regulation the cell adhesions, and by promote angiogenesis. MMP-13 may be involved in the carcinogenesis, MMP-2 and MMP-9 may be played some important roles in the degradation of ECM,BM. MMP-1 may be included in the degradation of ECM and also the modulation of cell adhesion.4. E-cadherin was down-regulated in lung cancer tissues,also the expression...
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