The Role And Significance Of The Alteration In DPC4 Gene And Transforming Growth Factor β Receptor Ⅰ, Ⅱ Gene In The Gastric Carcinomas

Object To investigate the expression of DPC4 (deleted in pancreatic carcinoma, locus 4) gene in normal gastric mucosa,chronic gastritis without entero- metaplasia and mucosa dysplasia, chronic atrophic gastritis with entero-metaplasia and mucosa dysplasia , gastric carcinoma as well as the expression of transforming growth factor P receptor I , II (TGF P R I , TGF P R II ) gene in grastric carcinoma.To study their biological significance in the occurrence and development of gastric carcinoma by means of these genie expressions.Methods Immunohistochemistry staining SABC (Strept-Avidin-Biotin Complex, SABC) was used to detect DPC4 expression in 10 normal gastric mucosa,10 chronic gastritis without entero- metaplasia and mucosa dysplasia,48 chronic atrophic gastritis with entero-metaplasia and mucosa dysplasia. Immunohistochemistry staining SABC was used to detect the expression of DPC4, TGF P R I , TGF P R II gene in 56 gastric carcinomas.Results l.The positive expression rate of DPC4 gene innormal gastric mucosa,chronic gastritis, chronic atrophic gastritis and gastric carcinoma is 100%, 100%,95.8% and 76.8% respectively.The significant difference was observed between gastric mucosa lesion and gastric carcinoma(P< 0. 05). 2. The positive expression rate of DPC4 gene in gastric carcinoma is 76. 8%, there is not significant difference among the histological classification, clinical Stagings and lymph nodal metastasis (P> 0. 05). 3. The positive expression rate of TGFP R II gene in gastric carcinoma is 30. 4%. But the expression rate in gastric carcinoma in higher and middle differentiation, earlier period,no lymph nodal metastasisis apparently higher, which are 75%, 71. 4%, 52. 9% respectively, than in lower differentiation, advanced period, lymph nodal metastasis,which are 18.2%,24. 5%, 20.5% respectively. There are significant differences among them(P<0.05orP<0.001) .4. The positive expression rate of TGF 3 R I gene in gastric carcinoma is 42.9%. But the expression rate in gastric carcinoma in higher and middle differentiation,earlier period.no lymph nodal metastasis is apparently higher .which are 75%,85.7%,70.6% respectively, than in lower differentiation, advanced period, lymph nodal metastasis, which are 34.1%,36.7%,30.8% respectively. There are significant differences among them(P<0. 05). 5. No significant difference is found in the positive expression in gastric carcinoma among the sex, tumor location and pathological type ( P > 0.05 ) .6. The positive expression of DPC4, TGF3RI , TGF 0 R II gene in gastric carcinoma are positive correlation (P<0. 05).CONCLUSION Gastric carcinoma presents alteration of DPC4 gene, TGFPRI gene and TGF P RII gene, which causedown-regulation of DPC4, TGF P R I , TGF & R II proteins. They are correlative with the occurrence, development and a lot of biological behavior of gastric carcinoma.The level of their expressions may be a sensitive predictor to judge the biological behavior of the gastric carcinoma in clinic.